Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] (ab208187)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19727] to PDK1 + PDK2 + PDK4
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human, Recombinant fragment

Overview

  • Product name

    Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727]
    See all PDK1 + PDK2 + PDK4 primary antibodies

  • Description

    Rabbit monoclonal [EPR19727] to PDK1 + PDK2 + PDK4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    Rat
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse PDK4, PDK1 and PDK2 recombinant proteins; mouse skeletal muscle, heart and liver lysates; C2C12 and HepG2 whole cell lysates; human and rat heart lysates; gastrocnemius muscle lysate from un-starved mouse and mouse starved for 72 hours; heart lysate from un-starved mouse and mouse starved for 72 hours. IP: Mouse skeletal muscle whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19727

  • Isotype

    IgG

Images

  • Western blot - Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] (ab208187)
    Western blot - Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] (ab208187)
    All lanes : Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] (ab208187) at 1/10000 dilution

    Lane 1 : Mouse PDK4 recombinant protein
    Lane 2 : Mouse PDK1 recombinant protein
    Lane 3 : Mouse PDK2 recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 47 kDa


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Mouse PDK4, PDK1 and PDK2 recombinant proteins contain aa8-412, aa1-434 and aa1-407 respectively with a His-Tag®.

  • Western blot - Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] (ab208187)
    Western blot - Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] (ab208187)
    All lanes : Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] (ab208187) at 1/1000 dilution

    Lane 1 : Mouse skeletal muscle lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse liver lysate
    Lane 4 : C2C12 (Mouse myoblast cell line) whole cell lysate
    Lane 5 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 6 : Human heart lysate
    Lane 7 : Rat heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 47 kDa
    Observed band size: 47 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1-4: 10 seconds; Lane 5-6:3 minutes; Lane 7: 2 seconds.

  • Immunoprecipitation - Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] (ab208187)
    Immunoprecipitation - Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] (ab208187)

    PDK1+PDK2+PDK4 was immunoprecipitated from 0.35 mg of Mouse skeletal muscle whole cell lysate with ab208187 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab208187 at 1/500 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Mouse skeletal muscle whole cell lysate 10µg (Input).

    Lane 2: ab208187 IP in Mouse skeletal muscle whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab208187 in Mouse skeletal muscle whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

  • Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] (ab208187)
    Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] (ab208187)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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