Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PDGF B antibody [326A] - BSA and Azide free (ab252808)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [326A] to PDGF B - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-PDGF B antibody [326A] - BSA and Azide free
    See all PDGF B primary antibodies

  • Description

    Rat monoclonal [326A] to PDGF B - BSA and Azide free

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human PDGF B. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P01127

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Human glioma, kidney and cerebrum tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab252808 is the carrier-free version of ab107101. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF B antibody [326A] - BSA and Azide free (ab252808)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF B antibody [326A] - BSA and Azide free (ab252808)

    Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling PDGF B with ab107101 at 2.56 µg/ml  followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on human glioma. The section was incubated with ab107101 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab107101).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF B antibody [326A] - BSA and Azide free (ab252808)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF B antibody [326A] - BSA and Azide free (ab252808)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling PDGF B with ab107101 at 2.56 µg/ml followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on glomerus of human kidney. The section was incubated with ab107101 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab107101).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF B antibody [326A] - BSA and Azide free (ab252808)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF B antibody [326A] - BSA and Azide free (ab252808)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling PDGF B with ab107101 at 2.56 µg/ml  followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on human cerebrum. The section was incubated with ab107101 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab107101).

  • Anti-PDGF B antibody [326A] - BSA and Azide free (ab252808)
    Anti-PDGF B antibody [326A] - BSA and Azide free (ab252808)

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