Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-PDE5A/PDE5 antibody [EPR24129-95] - BSA and Azide free
See all PDE5A/PDE5 primary antibodies
Description

Rabbit monoclonal [EPR24129-95] to PDE5A/PDE5 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: IP, IHC-Fr, IHC-P, WBmore details
Unsuitable for: Flow Cyt (Intra) or ICC
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human cerebellum, MCF7, Mouse esophagus, cerebellum, Rat cerebellum lysates. IHC-P: Human cerebellum and brain, Mouse cerebellum, Rat cerebellum. IHC-Fr: Mouse cerebellum, Rat cerebellum tissues. IP: MCF7 cells.
General notes
ab280891 is the carrier-free version of ab259945.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C.
Storage buffer

Constituent: 100% PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR24129-95
Isotype

IgG
Research areas

Western blot - Anti-PDE5A/PDE5 antibody [EPR24129-95] - BSA and Azide free (ab280891)

All lanes : Anti-PDE5A/PDE5 antibody [EPR24129-95] (ab259945) at 1/1000 dilution

Lane 1 : Human cerebellum tissue lysate
Lane 2 : Human liver tissue lysate
Lane 3 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate

Lysates/proteins at 40 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))

Predicted band size: 100 kDa

This data was developed using ab259945, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Negative control: liver (PMID: 11389965).

Exposure time: 37 seconds
Western blot - Anti-PDE5A/PDE5 antibody [EPR24129-95] - BSA and Azide free (ab280891)

All lanes : Anti-PDE5A/PDE5 antibody [EPR24129-95] (ab259945) at 1/1000 dilution

Lane 1 : Mouse esophagus tissue lysate
Lane 2 : Mouse cerebellum tissue lysate
Lane 3 : Mouse liver tissue lysate
Lane 4 : Rat cerebellum tissue lysate
Lane 5 : Rat liver tissue lysate

Lysates/proteins at 40 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 100 kDa

This data was developed using ab259945, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Negative control: liver (PMID: 11389965).

Exposure time: 37 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE5A/PDE5 antibody [EPR24129-95] - BSA and Azide free (ab280891)

This data was developed using ab259945, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labelling PDE5A/PDE5 with ab259945 at 1/500 (0.928 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining on purkinje cells of human cerebellum.The section was incubated with ab259945 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE5A/PDE5 antibody [EPR24129-95] - BSA and Azide free (ab280891)

This data was developed using ab259945, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling PDE5A/PDE5 with ab259945 at 1/500 (0.928 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining on artery smooth muscle of human brain.The section was incubated with ab259945 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE5A/PDE5 antibody [EPR24129-95] - BSA and Azide free (ab280891)

This data was developed using ab259945, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labelling PDE5A/PDE5 with ab259945 at 1/500 (0.928 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining on mouse cerebellum.The section was incubated with ab259945 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE5A/PDE5 antibody [EPR24129-95] - BSA and Azide free (ab280891)

This data was developed using ab259945, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat cerebellum tissue labelling PDE5A/PDE5 with ab259945 at 1/500 (0.928 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining on rat cerebellum.The section was incubated with ab259945 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE5A/PDE5 antibody [EPR24129-95] - BSA and Azide free (ab280891)

This data was developed using ab259945, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling PDE5A/PDE5 with ab259945 at 1/500 (0.928 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control: no staining on human liver.The section was incubated with ab259945 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Frozen sections) - Anti-PDE5A/PDE5 antibody [EPR24129-95] - BSA and Azide free (ab280891)

This data was developed using ab259945, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum tissue labeling PDE5A/PDE5 with ab259945 at 1/100 (4.64 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 ug/ml dilution (Green). Positive staining on mouse cerebellum is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Frozen sections) - Anti-PDE5A/PDE5 antibody [EPR24129-95] - BSA and Azide free (ab280891)

This data was developed using ab259945, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebellum tissue labeling PDE5A/PDE5 with ab259945 at 1/100 (4.64 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 ug/ml dilution (Green). Positive staining on rat cerebellum. is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 2 ug/ml dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunoprecipitation - Anti-PDE5A/PDE5 antibody [EPR24129-95] - BSA and Azide free (ab280891)

This data was developed using ab259945, the same antibody clone in a different buffer formulation.

PDE5A/PDE5 was immunoprecipitated from 0.35 mg MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 ug with ab259945 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259945 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 ug

Lane 2: ab259945 IP in MCF7 whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259945 in MCF7 whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 15 seconds
Anti-PDE5A/PDE5 antibody [EPR24129-95] - BSA and Azide free (ab280891)