Anti-PD-L1 antibody [ABM4E54] (ab210931)
![Anti-PD-L1 antibody [ABM4E54] (ab210931)](https://www.abcam.com/ps/products/210/ab210931/Images/ab210931-258484-anti-pd-l1-antibody-abm4e54-immunohistochemistry.jpg "Anti-PD-L1 antibody [ABM4E54] (ab210931)")
Key features and details
- Mouse monoclonal [ABM4E54] to PD-L1
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-PD-L1 antibody [ABM4E54]
See all PD-L1 primary antibodies -
Description
Mouse monoclonal [ABM4E54] to PD-L1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human PD-L1 aa 1-250.
Database link: Q9NZQ7-1 -
Positive control
- Recombinant protein fragment of Human PD-L1; Human Hodkin's lymphoma tissue. Programmed death-ligand 1 (PD-L1) expression in various tumor types - http://www.immunotherapyofcancer.org/content/1/S1/P53
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
ABM4E54 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [ABM4E54] (ab210931)
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human Hodkin's lymphoma tissue, labeling PD-L1 with ab210931at 5 µg/mL.
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![Western blot - Anti-PD-L1 antibody [ABM4E54] (ab210931)](https://www.abcam.com/ps/products/210/ab210931/Images/ab210931-258485-anti-pd-l1-antibody-abm4e54-western-blot.jpg)
Western blot - Anti-PD-L1 antibody [ABM4E54] (ab210931)Anti-PD-L1 antibody [ABM4E54] (ab210931) at 0.5 µg/ml + Recombinant protein fragment of Human PD-L1
Predicted band size: 33 kDa
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![Flow Cytometry - Anti-PD-L1 antibody [ABM4E54] (ab210931)](https://www.abcam.com/ps/products/210/ab210931/Images/ab210931-274648-anti-pd-l1-antibody-abm4e54-flow-cytometry.jpg)
Flow Cytometry - Anti-PD-L1 antibody [ABM4E54] (ab210931)Cell Surface flow cytometry analysis of PD-L1 in PHA treated human PBMC using 1 μg of ab210931. Green represents isotype control; pink represents ab210931 antibody. Goat anti-mouse PE conjugate was used as secondary antibody.
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![Western blot - Anti-PD-L1 antibody [ABM4E54] (ab210931)](https://www.abcam.com/ps/products/210/ab210931/Images/ab210931-285968-anti-pd-l1-antibody-abm4e54-western-blot.jpg)
Western blot - Anti-PD-L1 antibody [ABM4E54] (ab210931)All lanes : Anti-PD-L1 antibody [ABM4E54] (ab210931) at 2 µg/ml
Lane 1 : A549 lysate
Lane 2 : MCF-7 lysate
Lane 3 : 293 lysate
Lane 4 : HCT-116 lysate
Lane 5 : Saos2 lysate
Lane 6 : HeLa lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 33 kDaExposure time: 1 minute in film and 30 seconds in imager.
