Anti-PCYT2 antibody (ab135290)
Key features and details
- Rabbit polyclonal to PCYT2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PCYT2 antibody
See all PCYT2 primary antibodies -
Description
Rabbit polyclonal to PCYT2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human PCYT2 aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available asab157389) -
Positive control
- This antibody gave a positive signal in Human Liver tissue lysates as well as HepG2 whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab135290 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatHorseCowChimpanzeeMacaque monkeyChinese hamsterOrangutanApplication Abreviews Notes WB 1/250. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).Notes WB
1/250. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).Target
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Function
Plays an important role in the biosynthesis of the phospholipid phosphatidylethanolamine. Catalyzes the formation of CDP-ethanolamine. -
Tissue specificity
Strongest expression in liver, heart, and skeletal muscle. -
Pathway
Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 2/3. -
Sequence similarities
Belongs to the cytidylyltransferase family. - Information by UniProt
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Database links
- Entrez Gene: 5833 Human
- Entrez Gene: 68671 Mouse
- Entrez Gene: 89841 Rat
- SwissProt: Q99447 Human
- SwissProt: Q922E4 Mouse
- SwissProt: O88637 Rat
- Unigene: 569843 Human
- Unigene: 21439 Mouse
see all -
Alternative names
- CTP:phosphoethanolamine cytidylyltransferase antibody
- ET antibody
- Ethanolamine-phosphate cytidylyltransferase antibody
see all
Images
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Western blot - Anti-PCYT2 antibody (ab135290)All lanes : Anti-PCYT2 antibody (ab135290) at 1/250 dilution
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 44 kDa
Additional bands at: 16 kDa, 47 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab135290 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (1)
ab135290 has been referenced in 1 publication.
- Osawa T et al. Phosphoethanolamine Accumulation Protects Cancer Cells under Glutamine Starvation through Downregulation of PCYT2. Cell Rep 29:89-103.e7 (2019). PubMed: 31577958
Images
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Western blot - Anti-PCYT2 antibody (ab135290)All lanes : Anti-PCYT2 antibody (ab135290) at 1/250 dilution
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 44 kDa
Additional bands at: 16 kDa, 47 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab135290 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
