Anti-PBK/SPK antibody (ab226923)
Key features and details
- Rabbit polyclonal to PBK/SPK
- Suitable for: IP, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PBK/SPK antibody
See all PBK/SPK primary antibodies -
Description
Rabbit polyclonal to PBK/SPK -
Host species
Rabbit -
Tested applications
Suitable for: IP, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Chimpanzee -
Immunogen
Recombinant fragment within Human PBK/SPK (internal sequence). The exact sequence is proprietary.
Database link: Q96KB5 -
Positive control
- WB: HEK-293T, A431, HeLa, HepG2, U-87 MG, SK-N-SH, IMR32 and SK-N-AS whole cell extracts. ICC/IF: HeLa cells. IP: A431 whole cell extract.
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General notes
This product was previously labelled as PBK
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-PBK/SPK antibody (ab226923) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 36,37 kDa10% SDS-PAGE gel.
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4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling PBK/SPK (green) using ab226923 at 1/500 dilution in ICC/IF. Nuclear counterstained with Hoechst 33342 (blue).
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PBK/SPK was immunoprecipitated from A431 (human epidermoid carcinoma cell line) whole cell extract with 5 µg ab226923. Western blot was performed from the immunoprecipitate using ab226923. Anti-Rabbit IgG was used as a secondary antibody.
Lane 1: A431 whole cell extract (Input).
Lane 2: Control IgG IP in A431 whole cell extract.
Lane 3: ab226923 IP in A431 whole cell extract.
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All lanes : Anti-PBK/SPK antibody (ab226923) at 1/1000 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
Lane 3 : IMR32 whole cell extract
Lane 4 : SK-N-AS whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 36,37 kDa10% SDS-PAGE gel.