Antibodies, Proteins, Kits and Reagents for Life Science

526 012 ₸ Product size

100 µg 526 012 ₸ 1 mg 4 690 ₸

Order now and get it on Wednesday March 31, 2021

Anti-PAR2 antibody [EPR13675] - BSA and Azide free (ab240232)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13675] to PAR2 - BSA and Azide free
Suitable for: WB, ICC/IF, IHC-P, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-PAR2 antibody [EPR13675] - BSA and Azide free
See all PAR2 primary antibodies
Description

Rabbit monoclonal [EPR13675] to PAR2 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: WB, ICC/IF, IHC-P, Flow Cytmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- ICC: HepG2; IHC-P: Human, rat, and mouse kidney tissue sections; Flow Cyt: MCF-7 cell; WB: Human, rat and mouse kidney lysate, HePG2 whole cell lysate.
General notes
Ab240232 is the carrier-free version of ab180953. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab240232 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR13675
Isotype

IgG
Research areas

Western blot - Anti-PAR2 antibody [EPR13675] - BSA and Azide free (ab240232)

All lanes : Anti-PAR2 antibody [EPR13675] (ab180953) at 1/1000 dilution (Purified)

Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 2 : Rat kidney lysate

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 44 kDa

This data was developed using ab180953, the same antibody clone in a different buffer formulation.

We are unsure how to define the extra bands.
Western blot - Anti-PAR2 antibody [EPR13675] - BSA and Azide free (ab240232)

All lanes : Anti-PAR2 antibody [EPR13675] (ab180953) at 1/1000 dilution (Purified)

Lane 1 : Human kidney lysate
Lane 2 : Mouse kidney lysate

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 44 kDa

This data was developed using ab180953, the same antibody clone in a different buffer formulation.

We are unsure how to define the extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAR2 antibody [EPR13675] - BSA and Azide free (ab240232)

This data was developed using ab180953, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling PAR2 with Purified ab180953 at 1/100 dilution (1.38 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunocytochemistry - Anti-PAR2 antibody [EPR13675] - BSA and Azide free (ab240232)

This data was developed using ab180953, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling PAR2 with Purified ab180953 at 1/50 dilution (2.76 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 dilution (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 dilution (2 µg/mL). DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry - Anti-PAR2 antibody [EPR13675] - BSA and Azide free (ab240232)

This data was developed using ab180953, the same antibody clone in a different buffer formulation.

Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling PAR2 with unpurified ab180953 at 1/20 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAR2 antibody [EPR13675] - BSA and Azide free (ab240232)

This data was developed using ab180953, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling PAR2 with Purified ab180953 at 1/100 dilution (1.38 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAR2 antibody [EPR13675] - BSA and Azide free (ab240232)

This data was developed using ab180953, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling PAR2 with Purified ab180953 at 1/100 dilution (1.38 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Anti-PAR2 antibody [EPR13675] - BSA and Azide free (ab240232)

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