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Anti-p53 antibody [TP53/2092R] - BSA and Azide free (ab237922)

Anti-p53 antibody [TP53/2092R] - BSA and Azide free (ab237922)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [TP53/2092R] to p53 - BSA and Azide free
  • Suitable for: IHC-P, WB, Protein Array
  • Reacts with: Human

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Overview

  • Product name

    Anti-p53 antibody [TP53/2092R] - BSA and Azide free
    See all p53 primary antibodies
  • Description

    Rabbit monoclonal [TP53/2092R] to p53 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, Protein Arraymore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human p53. Recombinant human wild-type p53 protein
    Database link: P04637

  • Positive control

    • IHC-P: Human colon carcinoma tissue. WB: HeLa whole cell lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Ab purified by Protein A Column.
  • Clonality

    Monoclonal
  • Clone number

    TP53/2092R
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • p53
    • Cell Biology
    • Apoptosis
    • Intracellular
    • p53 Pathway
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • p53
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Cycle Inhibitors
    • p53
    • Cancer
    • Cell cycle
    • Cell cycle inhibitors
    • p53 pathway
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • p53 pathway

Images

  • Western blot - Anti-p53 antibody [TP53/2092R] - BSA and Azide free (ab237922)
    Western blot - Anti-p53 antibody [TP53/2092R] - BSA and Azide free (ab237922)
    Anti-p53 antibody [TP53/2092R] - BSA and Azide free (ab237922) at 1 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate.

    Predicted band size: 43 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 antibody [TP53/2092R] - BSA and Azide free (ab237922)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 antibody [TP53/2092R] - BSA and Azide free (ab237922)

    Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for p53 using ab237922 at 1 μg/mL in immunohistochemical analysis.

  • Protein Array - Anti-p53 antibody [TP53/2092R] - BSA and Azide free (ab237922)
    Protein Array - Anti-p53 antibody [TP53/2092R] - BSA and Azide free (ab237922)

    Analysis of Protein Array containing more than 19,000 full-length human proteins using ab237922.

    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

  • Anti-p53 antibody [TP53/2092R] - BSA and Azide free (ab237922)
    Anti-p53 antibody [TP53/2092R] - BSA and Azide free (ab237922)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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