Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-p53 antibody [SP5] - BSA and Azide free (ab242379)

Key features and details

  • Rabbit monoclonal [SP5] to p53 - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt, ICC/IF
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-p53 antibody [SP5] - BSA and Azide free
    See all p53 primary antibodies

  • Description

    Rabbit monoclonal [SP5] to p53 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein within Human p53. The exact sequence is proprietary.
    Database link: P04637

  • Positive control

    • IHC-P: Human colon adenocarcinoma, lung adenocarcinoma, stomach adenocarcinoma, ovarian adenocarcinoma, bladder transitional cell carcinoma, hepatocellular cell carcinoma, cervical squamous cell carcinoma, breast ductal cell carcinoma and B cell lymphoma tissue. Flow Cyt: HAP1 and MCF7 cells. ICC/IF: MCF7 cells.

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    ab242379 is the carrier-free version of ab16665. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab242379 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 antibody [SP5] - BSA and Azide free (ab242379)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 antibody [SP5] - BSA and Azide free (ab242379)

    Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for p53 using ab16665 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab16665).

  • Flow Cytometry - Anti-p53 antibody [SP5] - BSA and Azide free (ab242379)
    Flow Cytometry - Anti-p53 antibody [SP5] - BSA and Azide free (ab242379)

    This data was developed using the same antibody clone in a different buffer formulation (ab16665).

    Flow cytometry overlay histogram showing wild-type HAP1 (green line) and TP53 knockout HAP1 cells stained with ab16665 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1%PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab16665) (1x106 in 100 µl at 0.008 µg/ml) for 30 min at 22°C.

    The secondary antibody Goat anti-rabbit IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150081) was used at 1/2000 for 30 min at 22°C.

    Isotype control antibody was Rabbit IgG (monoclonal) (ab172730) used at the same concentration and conditions as the primary antibody (wild-type HAP1 - black line TP53 knockout HAP1 - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

    Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

    This antibody gave a positive signal in TP53 HAP1 knockout cells fixed with 80% methanol (5 min) / permeabilized with 0.1%PBS-Triton X-100 for 15 min used under the same conditions.

  • Flow Cytometry - Anti-p53 antibody [SP5] - BSA and Azide free (ab242379)
    Flow Cytometry - Anti-p53 antibody [SP5] - BSA and Azide free (ab242379)

    Flow cytometry analysis of MCF7 (human breast adenocarcinoma epithelial cell) labeling p53 with purified ab16665 at 1/200 dilution (0.81 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730)(black). Unlableled control - Unlabelled cells (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, sodium azide (ab16665).

  • Immunocytochemistry/ Immunofluorescence - Anti-p53 antibody [SP5] - BSA and Azide free (ab242379)
    Immunocytochemistry/ Immunofluorescence - Anti-p53 antibody [SP5] - BSA and Azide free (ab242379)

    Immunocytochemistry/ Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cell) cells labeling p53 with purified ab16665 at 1/25 (6.5 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, sodium azide (ab16665).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 antibody [SP5] - BSA and Azide free (ab242379)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 antibody [SP5] - BSA and Azide free (ab242379)

    Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for p53 using ab16665 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab16665).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 antibody [SP5] - BSA and Azide free (ab242379)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 antibody [SP5] - BSA and Azide free (ab242379)

    Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for p53 using ab16665 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab16665).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 antibody [SP5] - BSA and Azide free (ab242379)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 antibody [SP5] - BSA and Azide free (ab242379)

    Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for p53 using ab16665 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab16665).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 antibody [SP5] - BSA and Azide free (ab242379)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 antibody [SP5] - BSA and Azide free (ab242379)

    Formalin-fixed, paraffin-embedded human B cell lymphoma tissue stained for p53 using ab16665 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab16665).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 antibody [SP5] - BSA and Azide free (ab242379)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 antibody [SP5] - BSA and Azide free (ab242379)

    Formalin-fixed, paraffin-embedded human breast ductal cell carcinoma tissue stained for p53 using ab16665 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab16665).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 antibody [SP5] - BSA and Azide free (ab242379)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 antibody [SP5] - BSA and Azide free (ab242379)

    Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for p53 using ab16665 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, sodium azide (ab16665).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 antibody [SP5] - BSA and Azide free (ab242379)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 antibody [SP5] - BSA and Azide free (ab242379)

    Formalin-fixed, paraffin-embedded human hepatocellular cell carcinoma tissue stained for p53 using ab16665 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab16665).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 antibody [SP5] - BSA and Azide free (ab242379)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 antibody [SP5] - BSA and Azide free (ab242379)

    Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for p53 using ab16665 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab16665).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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