Anti-p53 antibody [EPR20416-117] - BSA and Azide free (ab252387)
![Anti-p53 antibody [EPR20416-117] - BSA and Azide free (ab252387)](https://www.abcam.com/ps/products/252/ab252387/Images/ab252387-1-211020IP1.jpg "Anti-p53 antibody [EPR20416-117] - BSA and Azide free (ab252387)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20416-117] to p53 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse
Overview
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Product name
Anti-p53 antibody [EPR20416-117] - BSA and Azide free
See all p53 primary antibodies -
Description
Rabbit monoclonal [EPR20416-117] to p53 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: LLC1 and RAW 264.7 whole cell lysate. IP: LLC1 whole cell lysate.
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General notes
Ab252387 is the carrier-free version of ab211020. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab252387 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20416-117 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-p53 antibody [EPR20416-117] - BSA and Azide free (ab252387)
p53 was immunoprecipitated from 0.35 mg LLC1 (mouse lung carcinoma cell line) whole cell lysate with ab211020 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab211020 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: LLC1 whole cell lysate 10 µg (Input).
Lane 2: ab211020 IP in LLC1 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211020 in LLC1 whole cell lysate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 30 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211020).
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![Anti-p53 antibody [EPR20416-117] - BSA and Azide free (ab252387)](https://www.abcam.com/ps/products/252/ab252387/Images/ab252387-2-benefits-of-recombinant-antibodies.png)
Anti-p53 antibody [EPR20416-117] - BSA and Azide free (ab252387)
