Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-p53 antibody [EPR20416-117] - BSA and Azide free (ab252387)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20416-117] to p53 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse

Overview

  • Product name

    Anti-p53 antibody [EPR20416-117] - BSA and Azide free
    See all p53 primary antibodies

  • Description

    Rabbit monoclonal [EPR20416-117] to p53 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: LLC1 and RAW 264.7 whole cell lysate. IP: LLC1 whole cell lysate.

  • General notes

    Ab252387 is the carrier-free version of ab211020. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab252387 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-p53 antibody [EPR20416-117] - BSA and Azide free (ab252387)
    Immunoprecipitation - Anti-p53 antibody [EPR20416-117] - BSA and Azide free (ab252387)

    p53 was immunoprecipitated from 0.35 mg LLC1 (mouse lung carcinoma cell line) whole cell lysate with ab211020 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab211020 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: LLC1 whole cell lysate 10 µg (Input). 

    Lane 2: ab211020 IP in LLC1 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211020 in LLC1 whole cell lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211020).

  • Anti-p53 antibody [EPR20416-117] - BSA and Azide free (ab252387)
    Anti-p53 antibody [EPR20416-117] - BSA and Azide free (ab252387)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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