Anti-P4HB antibody - C-terminal (ab223612)
Key features and details
- Rabbit polyclonal to P4HB - C-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-P4HB antibody - C-terminal
See all P4HB primary antibodies -
Description
Rabbit polyclonal to P4HB - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Rat P4HB aa 409-509 (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: P04785 -
Positive control
- WB: Rat brain, heart, kidney, liver, lung, pancreas, skeletal muscle, spleen, testis and thymus lysates; C2C12, NIH/3T3 and Pam212 cell lysates. ICC/IF: HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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2% formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for P4HB (yellow) using ab223612 at 1/100 dilution for 12 hours at 4°C in ICC/IF, followed by Goat Anti-Rabbit R-PE at 1/200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours at room temperature.
(A) DAPI (blue) nuclear stain (B) ab223612 (C) Merge.
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All lanes : Anti-P4HB antibody - C-terminal (ab223612) at 1/4000 dilution
Lane 1 : P4HB purified protein at 0.05 µg
Lane 2 : Rat brain lysate at 15 µg
Lane 3 : Rat heart lysate at 15 µg
Lane 4 : Rat kidney lysate at 15 µg
Lane 5 : Rat liver lysate at 15 µg
Lane 6 : Rat lung lysate at 15 µg
Lane 7 : Rat pancreas lysate at 15 µg
Lane 8 : Rat skeletal muscle lysate at 15 µg
Lane 9 : Rat spleen lysate at 15 µg
Lane 10 : Rat testis lysate at 15 µg
Lane 11 : Rat thymus lysate at 15 µg
Lane 12 : C2C12 (mouse myoblast cell line) cell lysate at 15 µg
Lane 13 : NIH/3T3 (mouse embyro fibroblast cell line) cell lysate at 15 µg
Lane 14 : Pam212 cell lysate at 15 µg
Secondary
All lanes : Donkey Anti-Rabbit IgG HRP
Predicted band size: 57 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
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2% formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for P4HB (green) using ab223612 at 1/100 dilution for 12 hours at 4°C in ICC/IF, followed by Goat Anti-Rabbit FITC at 1/200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours at room temperature.
(A) DAPI (blue) nuclear stain (B) ab223612 (C) Merge.