Anti-LEPRE1/P3H1 antibody [EPR10193(B)] - BSA and Azide free (ab249107)
![Anti-LEPRE1/P3H1 antibody [EPR10193(B)] - BSA and Azide free (ab249107)](https://www.abcam.com/ps/products/249/ab249107/Images/ab249107-408072-anti-lepre1-p3h1-antibody-epr10193-b-bsa-and-azide-free-western-blot.jpg "Anti-LEPRE1/P3H1 antibody [EPR10193(B)] - BSA and Azide free (ab249107)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10193(B)] to LEPRE1/P3H1 - BSA and Azide free
- Suitable for: ICC, WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-LEPRE1/P3H1 antibody [EPR10193(B)] - BSA and Azide free
See all LEPRE1/P3H1 primary antibodies -
Description
Rabbit monoclonal [EPR10193(B)] to LEPRE1/P3H1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WB, Flow Cytmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab249107 is the carrier-free version of ab154799 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab249107 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as LEPRE1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR10193(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] - BSA and Azide free (ab249107)All lanes : Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : A549 cell lysate
Lane 4 : Human placenta tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 83 kDaThis data was developed using ab154799, the same antibody clone in a different buffer formulation.
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![Immunocytochemistry - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] - BSA and Azide free (ab249107)](https://www.abcam.com/ps/products/249/ab249107/Images/ab249107-2-anti-lepre1-p3h1-antibody-epr10193-b-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] - BSA and Azide free (ab249107)This data was developed using ab154799, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling LEPRE1/P3H1 with Purified ab154799 at 1/500 dilution (5µg/ml). Cells were fixed with 100% methanol. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control. -
![Immunocytochemistry - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] - BSA and Azide free (ab249107)](https://www.abcam.com/ps/products/249/ab249107/Images/ab249107-1-anti-lepre1-p3h1-antibody-epr10193-b-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] - BSA and Azide free (ab249107) -
![Anti-LEPRE1/P3H1 antibody [EPR10193(B)] - BSA and Azide free (ab249107)](https://www.abcam.com/ps/products/249/ab249107/Images/ab249107-4-benefits-of-recombinant-antibodies.png)
Anti-LEPRE1/P3H1 antibody [EPR10193(B)] - BSA and Azide free (ab249107)