Anti-p35 antibody (ab235271)
Key features and details
- Rabbit polyclonal to p35
- Suitable for: IHC, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-p35 antibody
See all p35 primary antibodies -
Description
Rabbit polyclonal to p35 -
Host species
Rabbit -
Tested applications
Suitable for: IHC, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Human p35 overexpressing HEK-293T lysate, C-terminal DDK tag. Mouse brain lysate. IHC-P: Mouse brain tissue.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Mouse brain tissue stained for p35 using ab235271 at 1/100 dilution in immunohistochemical analysis. Counterstained with hematoxylin.
HIER antigen retrieval.
ab235271 developed a specific staining of neurons and neurophil in brain tissue.
-
Anti-p35 antibody (ab235271) at 1/1000 dilution + Mouse brain lysate at 35 µg
Secondary
Donkey anti Rabbit IgG peroxidase conjugate at 1/70000 dilution
Predicted band size: 34 kDa
Exposure time: 10 seconds
-
All lanes : Anti-p35 antibody (ab235271) at 1/1000 dilution
Lane 1 : Human CDK5 overexpressing HEK293T lysate
Lane 2 : Human CDK5 empty vector HEK293T lysate
Lane 3 : NIH/3T3 (mouse embyro fibroblast cell line) lysate
Secondary
All lanes : Goat-anti-Rabbit IgG [H&L] at 1/70000 dilution
Predicted band size: 34 kDa
Exposure time: 30 seconds