Anti-P2X7 antibody (ab109054)
Key features and details
- Rabbit polyclonal to P2X7
- Suitable for: WB, IHC-P
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-P2X7 antibody
See all P2X7 primary antibodies -
Description
Rabbit polyclonal to P2X7 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P RatWB Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in Rat Hippocampus tissue lysate in western blot, and FFPE rat brain tissue sections in IHCP.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109054 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species IHC-P RatWB RatAll applications MouseApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).IHC-P Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).IHC-P
Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.Target
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Function
Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. -
Tissue specificity
Widely expressed with highest levels in brain and immune tissues. -
Sequence similarities
Belongs to the P2X receptor family. -
Post-translational
modificationsPhosphorylation results in its inactivation.
ADP-ribosylation at Arg-125 is necessary and sufficient to activate P2RX7 and gate the channel.
Palmitoylation of several cysteines in the C-terminal cytoplasmic tail is required for efficient localization to cell surface. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 18439 Mouse
- Entrez Gene: 29665 Rat
- SwissProt: Q9Z1M0 Mouse
- SwissProt: Q64663 Rat
- Unigene: 42026 Mouse
- Unigene: 10510 Rat
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Alternative names
- ATP receptor antibody
- P2rx7 antibody
- P2RX7_HUMAN antibody
see all
Images
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Western blot - Anti-P2X7 antibody (ab109054)Anti-P2X7 antibody (ab109054) at 1 µg/ml + Rat Hippocampus Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 68 kDa
Additional bands at: 20 kDa, 78 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody (ab109054)IHC image of ab109054 staining in rat brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab109054, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
References (2)
ab109054 has been referenced in 2 publications.
- Liu Y et al. The P2X7 receptor (P2X7R)-specific antagonist A804598 inhibits inflammatory reaction in human fibroblast-like synoviocytes. Am J Transl Res 12:45-53 (2020). PubMed: 32051736
- Yang J et al. High glucose inhibits osteogenic differentiation and proliferation of MC3T3-E1 cells by regulating P2X7. Mol Med Rep 20:5084-5090 (2019). PubMed: 31702818
Images
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Western blot - Anti-P2X7 antibody (ab109054)Anti-P2X7 antibody (ab109054) at 1 µg/ml + Rat Hippocampus Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 68 kDa
Additional bands at: 20 kDa, 78 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2X7 antibody (ab109054)IHC image of ab109054 staining in rat brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab109054, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
