Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸ 10 µl 127 315 ₸

Order now and get it on Tuesday March 09, 2021

Anti-p150 CAF1/CAF antibody [EPR5576(2)] (ab126625)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR5576(2)] to p150 CAF1/CAF
Suitable for: WB, IP, IHC-P, Flow Cyt
Reacts with: Human

Product name

Anti-p150 CAF1/CAF antibody [EPR5576(2)]
See all p150 CAF1/CAF primary antibodies
Description

Rabbit monoclonal [EPR5576(2)] to p150 CAF1/CAF
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human p150 CAF1/CAF aa 100-200. The exact sequence is proprietary.
Positive control
- Jurkat, HeLa, HeLa treated with hydroxyurea and K562 whole cell lysates; Human colon and tonsil tissues.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as p150 CAF1

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR5576(2)
Isotype

IgG
Research areas

Western blot - Anti-p150 CAF1/CAF antibody [EPR5576(2)] (ab126625)

All lanes : Anti-p150 CAF1/CAF antibody [EPR5576(2)] (ab126625) at 1/1000 dilution

Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate, treated with hydroxyourea
Lane 3 : HeLa cell line lysate
Lane 4 : K562 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 107 kDa
Flow Cytometry - Anti-p150 CAF1/CAF antibody [EPR5576(2)] (ab126625)

Overlay histogram showing HeLa cells stained with ab126625 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126625, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p150 CAF1/CAF antibody [EPR5576(2)] (ab126625)

Formalin-fixed, paraffin-embedded human colon tissue stained for p150 CAF1/CAF with ab126625 (1/100 dilution) in immunohistochemical analysis.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p150 CAF1/CAF antibody [EPR5576(2)] (ab126625)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for p150 CAF1/CAF with ab126625 (1/100 dilution) in immunohistochemical analysis.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-p150 CAF1/CAF antibody [EPR5576(2)] (ab126625)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com