Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-p150 CAF1/CAF antibody [EPR5576(2)] (ab126625)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5576(2)] to p150 CAF1/CAF
  • Suitable for: WB, IP, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-p150 CAF1/CAF antibody [EPR5576(2)]
    See all p150 CAF1/CAF primary antibodies

  • Description

    Rabbit monoclonal [EPR5576(2)] to p150 CAF1/CAF

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human p150 CAF1/CAF aa 100-200. The exact sequence is proprietary.

  • Positive control

    • Jurkat, HeLa, HeLa treated with hydroxyurea and K562 whole cell lysates; Human colon and tonsil tissues.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     This product was previously labelled as p150 CAF1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-p150 CAF1/CAF antibody [EPR5576(2)] (ab126625)
    Western blot - Anti-p150 CAF1/CAF antibody [EPR5576(2)] (ab126625)
    All lanes : Anti-p150 CAF1/CAF antibody [EPR5576(2)] (ab126625) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate, treated with hydroxyourea
    Lane 3 : HeLa cell line lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 107 kDa

  • Flow Cytometry - Anti-p150 CAF1/CAF antibody [EPR5576(2)] (ab126625)
    Flow Cytometry - Anti-p150 CAF1/CAF antibody [EPR5576(2)] (ab126625)
    Overlay histogram showing HeLa cells stained with ab126625 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126625, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p150 CAF1/CAF antibody [EPR5576(2)] (ab126625)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p150 CAF1/CAF antibody [EPR5576(2)] (ab126625)

    Formalin-fixed, paraffin-embedded human colon tissue stained for p150 CAF1/CAF with ab126625 (1/100 dilution) in immunohistochemical analysis.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p150 CAF1/CAF antibody [EPR5576(2)] (ab126625)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p150 CAF1/CAF antibody [EPR5576(2)] (ab126625)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for p150 CAF1/CAF with ab126625 (1/100 dilution) in immunohistochemical analysis.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-p150 CAF1/CAF antibody [EPR5576(2)] (ab126625)
    Anti-p150 CAF1/CAF antibody [EPR5576(2)] (ab126625)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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