Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free (ab250456)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13354] to ANP32C/PP32R1 - BSA and Azide free
  • Suitable for: ICC, IP, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free
    See all ANP32C/PP32R1 primary antibodies

  • Description

    Rabbit monoclonal [EPR13354] to ANP32C/PP32R1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IP, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250456 is the carrier-free version of ab181242 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab250456 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as ANP32C

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13354

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free (ab250456)
    Western blot - Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free (ab250456)
    All lanes : Anti-ANP32C/PP32R1 antibody [EPR13354] (ab181242) at 1/10000 dilution

    Lane 1 : HUVEC cell lysate
    Lane 2 : PC-3 cell lysate
    Lane 3 : BxPC-3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 27 kDa
    Observed band size: 27 kDa



    This data was developed using ab181242, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free (ab250456)
    Immunocytochemistry - Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free (ab250456)
    This data was developed using ab181242, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed PC-3 cells labeling ANP32C/PP32R1 with ab181242 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
  • Flow Cytometry - Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free (ab250456)
    Flow Cytometry - Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free (ab250456)
    This data was developed using ab181242, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed MCF-7 cells labeling ANP32C/PP32R1 with ab181242 at 1/20 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
  • Immunoprecipitation - Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free (ab250456)
    Immunoprecipitation - Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free (ab250456)

    This data was developed using ab181242, the same antibody clone in a different buffer formulation.

    Western blot analysis of ANP32C/PP32R1 in MCF-7 cell lysate immunoprecipitated with ab181242 at 1/50 dilution.

     Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free (ab250456)
    Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free (ab250456)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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