Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free (ab250456)
![Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free (ab250456)](https://www.abcam.com/ps/products/250/ab250456/Images/ab250456-407863-anti-anp32c-pp32r1-antibody-epr13354-bsa-and-azide-free-western-blot.jpg "Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free (ab250456)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13354] to ANP32C/PP32R1 - BSA and Azide free
- Suitable for: ICC, IP, Flow Cyt, WB
- Reacts with: Human
Overview
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Product name
Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free
See all ANP32C/PP32R1 primary antibodies -
Description
Rabbit monoclonal [EPR13354] to ANP32C/PP32R1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IP, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250456 is the carrier-free version of ab181242 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab250456 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as ANP32C
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR13354 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free (ab250456)All lanes : Anti-ANP32C/PP32R1 antibody [EPR13354] (ab181242) at 1/10000 dilution
Lane 1 : HUVEC cell lysate
Lane 2 : PC-3 cell lysate
Lane 3 : BxPC-3 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDaThis data was developed using ab181242, the same antibody clone in a different buffer formulation.
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![Immunocytochemistry - Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free (ab250456)](https://www.abcam.com/ps/products/250/ab250456/Images/ab250456-3-anti-anp32c-pp32r1-antibody-epr13354-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free (ab250456)This data was developed using ab181242, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed PC-3 cells labeling ANP32C/PP32R1 with ab181242 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue). -
![Flow Cytometry - Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free (ab250456)](https://www.abcam.com/ps/products/250/ab250456/Images/ab250456-2-anti-anp32c-pp32r1-antibody-epr13354-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free (ab250456)This data was developed using ab181242, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed MCF-7 cells labeling ANP32C/PP32R1 with ab181242 at 1/20 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution. -
![Immunoprecipitation - Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free (ab250456)](https://www.abcam.com/ps/products/250/ab250456/Images/ab250456-399621-anti-anp32c-pp32r1-antibody-epr13354-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free (ab250456)This data was developed using ab181242, the same antibody clone in a different buffer formulation.
Western blot analysis of ANP32C/PP32R1 in MCF-7 cell lysate immunoprecipitated with ab181242 at 1/50 dilution.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
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![Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free (ab250456)](https://www.abcam.com/ps/products/250/ab250456/Images/ab250456-5-benefits-of-recombinant-antibodies.png)
Anti-ANP32C/PP32R1 antibody [EPR13354] - BSA and Azide free (ab250456)
