Anti-p115-RhoGEF antibody (ab223759)
Key features and details
- Rabbit polyclonal to p115-RhoGEF
- Suitable for: ICC, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-p115-RhoGEF antibody
See all p115-RhoGEF primary antibodies -
Description
Rabbit polyclonal to p115-RhoGEF -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human p115-RhoGEF aa 200-350.
Database link: Q92888 -
Positive control
- WB: RT4, U-251 MG sp, NIH/3T3 and NBT-II cell lysates. IHC: Human lymph node, small intestine and lung tissue. ICC: U-2 OS cells.
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General notes
Previously labelled as ARHGEF1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p115-RhoGEF antibody (ab223759)
Paraffin embedded sections of human small intestine tissue stained for p115-RhoGEF with ab223759 at 1/1000 dilution in immunohistochemical analysis.
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Immunocytochemistry - Anti-p115-RhoGEF antibody (ab223759)PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for p115-RhoGEF (green) using ab223759 at 4 µg/ml in ICC.
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Western blot - Anti-p115-RhoGEF antibody (ab223759)All lanes : Anti-p115-RhoGEF antibody (ab223759) at 1/100 dilution
Lane 1 : NIH/3T3 (mouse embyro fibroblast cell line) cell lysate
Lane 2 : NBT-II cell lysate
Predicted band size: 102.4 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p115-RhoGEF antibody (ab223759)Paraffin embedded sections of human lymph node tissue stained for p115-RhoGEF with ab223759 at 1/1000 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p115-RhoGEF antibody (ab223759)Paraffin embedded sections of human lung tissue stained for p115-RhoGEF with ab223759 at 1/1000 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p115-RhoGEF antibody (ab223759)Paraffin embedded sections of human liver tissue stained for p115-RhoGEF with ab223759 at 1/1000 dilution in immunohistochemical analysis. No positivity observed.
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Western blot - Anti-p115-RhoGEF antibody (ab223759)All lanes : Anti-p115-RhoGEF antibody (ab223759) at 1/100 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG sp cell lysate
Predicted band size: 102.4 kDa
