Anti-OX40L/TNFSF4 antibody [EP1168Y] (ab76130)
Key features and details
- Rabbit monoclonal [EP1168Y] to OX40L/TNFSF4
- Suitable for: WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-OX40L/TNFSF4 antibody [EP1168Y]
See all OX40L/TNFSF4 primary antibodies -
Description
Rabbit monoclonal [EP1168Y] to OX40L/TNFSF4 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Synthetic peptide within Human CD252/OX40L (C terminal). The exact sequence is proprietary.
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Positive control
- WB: HUVEC and A-172 whole cell lysate. Recombinant human CD252 / OX40L.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP1168Y -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-OX40L/TNFSF4 antibody [EP1168Y] (ab76130) at 1/1000 dilution
Lane 1 : HUVEC (human umbilical vein endothelial cell) whole cell lysate
Lane 2 : A-172 (human brain glioblastoma) whole cell lysate
Lane 3 : Raji (human burkitt's lymphoma b lymphocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa
Exposure time: 6 secondsOX40L/TNFSF4 is a glycoprotein. The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID: 8642328)
Negative control: Raji (PMID: 8642328)
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All lanes : Anti-OX40L/TNFSF4 antibody [EP1168Y] (ab76130) at 1/50000 dilution
Lane 1 : Recombinant human CD252 / OX40L at 0.01 µg
Lane 2 : Recombinant human CD252 / OX40L at 0.005 µg
Secondary
All lanes : goat anti-rabbit-HRP at 1/1000 dilution
Predicted band size: 30 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?The recombinant protein was expressed as a fusion protein.