Anti-CD45 antibody [MRC OX-1] - BSA and Azide free (ab244568)
![Anti-CD45 antibody [MRC OX-1] - BSA and Azide free (ab244568)](https://www.abcam.com/ps/products/244/ab244568/Images/ab244568-2-ab33923IHCFr2.jpg "Anti-CD45 antibody [MRC OX-1] - BSA and Azide free (ab244568)")
Key features and details
- Mouse monoclonal [MRC OX-1] to CD45 - BSA and Azide free
- Suitable for: IHC-Fr
- Reacts with: Mouse, Rat
- Isotype: IgG1
Overview
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Product name
Anti-CD45 antibody [MRC OX-1] - BSA and Azide free
See all CD45 primary antibodies -
Description
Mouse monoclonal [MRC OX-1] to CD45 - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-Fr MouseRat
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Immunogen
Tissue, cells or virus corresponding to Rat CD45. Rat thymocyte membrane glycoproteins.
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Positive control
- IHC-Fr: Mouse and rat spleen tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
MRC OX-1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Frozen sections) - Anti-CD45 antibody [MRC OX-1] - BSA and Azide free (ab244568)
IHC image of CD45 staining in a section of frozen normal rat spleen*.
The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining.
Non-specific protein-protein interactions were blocked using TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1 hour at room temperature. The section was then incubated with ab33923 (1µg/ml dilution) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then incubated with ab150119 ((Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647) (shown in red) and DAPI (staining nuclear DNA) (shown in blue) for 1 hour at room temperature. The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor® 647 signal is derived directly from bound ab33923.
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
*Tissue obtained from Charles River.
This data was developed using the same antibody clone in a different buffer formulation containing PBS and Sodium Azide (ab33923)
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![Immunohistochemistry (Frozen sections) - Anti-CD45 antibody [MRC OX-1] - BSA and Azide free (ab244568)](https://www.abcam.com/ps/products/244/ab244568/Images/ab244568-1-ab33923IHCFr1.jpg)
Immunohistochemistry (Frozen sections) - Anti-CD45 antibody [MRC OX-1] - BSA and Azide free (ab244568)IHC image of CD45 staining in a section of frozen normal mouse spleen*.
The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining.
Non-specific protein-protein interactions were blocked using the rodent block from Mouse on Mouse detection kit ab127055 for 1 hour at room temperature. The section was then incubated with ab33923 (1µg/ml dilution) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then incubated with ab150119 ((Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647) (shown in red) and DAPI (staining nuclear DNA) (shown in blue) for 1 hour at room temperature. The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor® 647 signal is derived directly from bound ab33923.
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
*Tissue obtained from Charles River.
This data was developed using the same antibody clone in a different buffer formulation containing PBS and Sodium Azide (ab33923)
