Antibodies, Proteins, Kits and Reagents for Life Science

251 280 ₸ Product size

100 µl 251 280 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR8646] to ORP1
Suitable for: ICC/IF, WB, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-ORP1 antibody [EPR8646]
See all ORP1 primary antibodies
Description

Rabbit monoclonal [EPR8646] to ORP1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

corresponding to Human ORP1 aa 900-1000.
Positive control
- WB: A549, 293T, U87-MG cell lysates; Mouse brain and heart lysates; Rat brain lysates Flow Cyt: A549 cells ICC/Flow Cyt: A549 and U87-MG cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR8646
Isotype

IgG
Research areas

Western blot - Anti-ORP1 antibody [EPR8646] (ab131165)

All lanes : Anti-ORP1 antibody [EPR8646] (ab131165) at 1/10000 dilution (Purified)

Lane 1 : A549 (Human lung carcinoma epithelial cell) whole cell lysates
Lane 2 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates
Lane 3 : Mouse brain lysates
Lane 4 : Mouse heart lysates
Lane 5 : Rat brain lysates
Lane 6 : Rat heart lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 108 kDa
Observed band size: 108,50 kDa
why is the actual band size different from the predicted?

Two mRNA variants produce a 108 kDa long ORP1 (ORP1L) and a 50 KDa short ORP1 (ORP1S) separately, which is consistent with what has been described in PMID: 12631712.
Western blot - Anti-ORP1 antibody [EPR8646] (ab131165)

Anti-ORP1 antibody [EPR8646] (ab131165) at 1/1000 dilution + 293T (Human embryonic kidney epithelial cell) whole cell lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 108 kDa
Observed band size: 108,50 kDa why is the actual band size different from the predicted?

Two mRNA variants produce a 108 kDa long ORP1 (ORP1L) and a 50 KDa short ORP1 (ORP1S) separately, which is consistent with what has been described in PMID: 12631712.
Flow Cytometry - Anti-ORP1 antibody [EPR8646] (ab131165)

Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labeling ORP1 with purified ab131165 at 1/60 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunocytochemistry/ Immunofluorescence - Anti-ORP1 antibody [EPR8646] (ab131165)

Immunocytochemistry/ Immunofluorescence analysis of A549 (Human lung carcinoma epithelial cell) cells labeling ORP1 with purified ab131165 at 1:200 dilution (2.8 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Western blot - Anti-ORP1 antibody [EPR8646] (ab131165)

All lanes : Anti-ORP1 antibody [EPR8646] (ab131165) at 1/1000 dilution (unpurified)

Lane 1 : A549 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : U87-MG cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 108 kDa
Observed band size: 50-108 kDa why is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Anti-ORP1 antibody [EPR8646] (ab131165)

ab131165 (unpurified) staining ORP1 in U87-MG (human glioblastoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000.

DAPI was used as a nuclear counterstain and the negative control was PBS only.
Flow Cytometry - Anti-ORP1 antibody [EPR8646] (ab131165)

ab131165 (unpurified) at 1/10 dilution staining ORP1 in permeabilized A549 cells by Flow cytometry (red). Rabbit IgG negative control (green).
Anti-ORP1 antibody [EPR8646] (ab131165)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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