Anti-Nup107 antibody [EPR22877-21] - BSA and Azide free (ab256530)
![Anti-Nup107 antibody [EPR22877-21] - BSA and Azide free (ab256530)](https://www.abcam.com/ps/products/256/ab256530/Images/ab256530-1-IP1anti-nup107-epr22868-87-immunoprecipitation-293t-human.jpg "Anti-Nup107 antibody [EPR22877-21] - BSA and Azide free (ab256530)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22877-21] to Nup107 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Nup107 antibody [EPR22877-21] - BSA and Azide free
See all Nup107 primary antibodies -
Description
Rabbit monoclonal [EPR22877-21] to Nup107 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: PC-12, RAW 264.7, NIH/3T3, HepG2, HEK-293T and HeLa whole cell lysates. IP: HEK-293T whole cell lysate.
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General notes
Ab256530 is the carrier-free version of ab236634. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab256530 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22877-21 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-Nup107 antibody [EPR22877-21] - BSA and Azide free (ab256530)
Nup107 was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney epithelial cell) whole cell lysate 10ug with ab236634 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab236634 at 1/1000. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: HEK-293T (human embryonic kidney epithelial cell) whole cell lysate 10ug
Lane 2: ab236634 IP in HEK-293T whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab236634 in HEK-293T whole cell lysate.
Blocking and dilution buffer and concentration/ 5% NFDM/TBST.
Exposure time: 6 seconds
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236634).
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![Anti-Nup107 antibody [EPR22877-21] - BSA and Azide free (ab256530)](https://www.abcam.com/ps/products/256/ab256530/Images/ab256530-2-benefits-of-recombinant-antibodies.png)
Anti-Nup107 antibody [EPR22877-21] - BSA and Azide free (ab256530)
