Anti-Nup107 antibody [EPR22877-21] (ab236634)
![Anti-Nup107 antibody [EPR22877-21] (ab236634)](https://www.abcam.com/ps/products/236/ab236634/Images/ab236634-352744-WB2anti-nup107-epr22868-87-western-blot-mouse-rat.jpg "Anti-Nup107 antibody [EPR22877-21] (ab236634)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22877-21] to Nup107
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Nup107 antibody [EPR22877-21]
See all Nup107 primary antibodies -
Description
Rabbit monoclonal [EPR22877-21] to Nup107 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB MouseRatHuman
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: PC-12, RAW 264.7, NIH/3T3, HepG2, HEK-293T and HeLa whole cell lysates. IP: HEK-293T whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22877-21 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Nup107 antibody [EPR22877-21] (ab236634)All lanes : Anti-Nup107 antibody [EPR22877-21] (ab236634) at 1/1000 dilution (Purified)
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
Lane 2 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 106 kDa
Observed band size: 107 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsThis antibody reacts with an unidentifiable protein around 37 kDa.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Nup107 antibody [EPR22877-21] (ab236634)](https://www.abcam.com/ps/products/236/ab236634/Images/ab236634-352732-WB1anti-nup107-epr22868-87-western-blot-human.jpg)
Western blot - Anti-Nup107 antibody [EPR22877-21] (ab236634)All lanes : Anti-Nup107 antibody [EPR22877-21] (ab236634) at 1/1000 dilution (Purified)
Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 10 µg
Lane 2 : 293T (human embryonic kidney epithelial cell), whole cell lysate at 10 µg
Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 106 kDa
Observed band size: 107 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times:
Lanes 1-2: 3 minutes; Lane 3: 15 seconds.
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![Immunoprecipitation - Anti-Nup107 antibody [EPR22877-21] (ab236634)](https://www.abcam.com/ps/products/236/ab236634/Images/ab236634-1-IP1anti-nup107-epr22868-87-immunoprecipitation-293t-human.jpg)
Immunoprecipitation - Anti-Nup107 antibody [EPR22877-21] (ab236634)Nup107 was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney epithelial cell) whole cell lysate 10ug with ab236634 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab236634. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: HEK-293T (human embryonic kidney epithelial cell) whole cell lysate 10ug
Lane 2: ab236634 IP in HEK-293T whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab236634 in HEK-293T whole cell lysate.
Blocking and dilution buffer and concentration/ 5% NFDM/TBST.
Exposure time: 6 seconds
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![Anti-Nup107 antibody [EPR22877-21] (ab236634)](https://www.abcam.com/ps/products/236/ab236634/Images/ab236634-5-benefits-of-recombinant-antibodies.png)
Anti-Nup107 antibody [EPR22877-21] (ab236634)
