Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NTF2 antibody [5A3] (ab254146)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [5A3] to NTF2
  • Suitable for: WB, ICC
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-NTF2 antibody [5A3]
    See all NTF2 primary antibodies

  • Description

    Mouse monoclonal [5A3] to NTF2

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HEK-293, HepG2, K562, IMR-32, F9 and L6 whole cell lysate. ICC: HeLa cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    5A3

  • Isotype

    IgG2

  • Research areas

Images

  • Western blot - Anti-NTF2 antibody [5A3] (ab254146)
    Western blot - Anti-NTF2 antibody [5A3] (ab254146)
    All lanes : Anti-NTF2 antibody [5A3] (ab254146) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : HEK-293 (human embryonic kidney epithelial cell), whole cell lysate
    Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
    Lane 4 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
    Lane 5 : IMR-32 (human neuroblastoma neuroblast), whole cell lysate
    Lane 6 : F9 (mouse embryonal carcinoma epithelial cell), whole cell lysate
    Lane 7 : L6 (rat skeletal muscle myoblast), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 14 kDa


    Exposure time: 8 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-NTF2 antibody [5A3] (ab254146)
    Immunocytochemistry - Anti-NTF2 antibody [5A3] (ab254146)

    Immunocytochemical analysis of 4% paraformaldehyde-fixed, 100% methanol-permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling NTF2 with ab254146 at 1/50 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and cytoplasmic staining in HeLa cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).
    Negative control 1: ab254146 at a 1/50 dilution followed by ab150080 at a 1/200 dilution.
    Negative control 2: ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.

  • Anti-NTF2 antibody [5A3] (ab254146)
    Anti-NTF2 antibody [5A3] (ab254146)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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