Anti-NRXN2 antibody (ab34245)
Key features and details
- Rabbit polyclonal to NRXN2
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NRXN2 antibody -
Description
Rabbit polyclonal to NRXN2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
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Positive control
- normal adult human cerebral cortex SKNSH cell line
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General notes
This product was previously labelled as Neurexin II alpha
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab34245 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanAll applications MouseRatApplication Abreviews Notes ICC/IF Use a concentration of 5 µg/ml.Notes ICC/IF
Use a concentration of 5 µg/ml.Target
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Relevance
Neurexin 2 alpha belongs to the Neurexin family, a group of neuronal proteins that function as cell adhesion molecules during synaptogenesis and intracellular signaling. Mammalian genomes contain just three neurexin genes. However, many (>200) neurexin isoforms may be expressed through the use of two alternative promoters and alternative splicing at up to five different positions. -
Cellular localization
Membrane; single-pass type I membrane protein. -
Database links
- Entrez Gene: 9379 Human
- Entrez Gene: 18190 Mouse
- Entrez Gene: 116595 Rat
- SwissProt: Q9P2S2 Human
- SwissProt: Q63374 Rat
- Unigene: 372938 Human
- Unigene: 329616 Mouse
- Unigene: 414022 Mouse
see all -
Alternative names
- Neurexin 2 antibody
- Neurexin II antibody
- NRXN2 antibody
Images
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Western blot - Anti-NRXN2 antibody (ab34245)Image courtesy of an anonymous Abreview.Anti-NRXN2 antibody (ab34245) at 1/400 dilution + whole cell lysate prepared from murine N2A cells at 20 µg
Secondary
Goat polyclonal conjugated to Dylight 800 at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 185 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
Samples were blocked using 0.2% casein for 1 hour at 37°C. -
Immunocytochemistry/ Immunofluorescence - Anti-NRXN2 antibody (ab34245)ICC/IF image of ab34245 stained SKNSH cells. The cells were 4% formaldehdye fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab34245, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (4)
ab34245 has been referenced in 4 publications.
- Harkin LF et al. Neurexins 1-3 Each Have a Distinct Pattern of Expression in the Early Developing Human Cerebral Cortex. Cereb Cortex 27:216-232 (2017). PubMed: 28013231
- Brito-Moreira J et al. Interaction of amyloid-ß (Aß) oligomers with neurexin 2a and neuroligin 1 mediates synapse damage and memory loss in mice. J Biol Chem 292:7327-7337 (2017). PubMed: 28283575
- Shapiro-Reznik M et al. Diurnal rhythms in neurexins transcripts and inhibitory/excitatory synapse scaffold proteins in the biological clock. PLoS One 7:e37894 (2012). WB ; Rat . PubMed: 22662246
- Borsics T et al. Subcellular distribution and expression of prenylated Rab acceptor 1 domain family, member 2 (PRAF2) in malignant glioma: Influence on cell survival and migration. Cancer Sci : (2010). IHC-P ; Human . PubMed: 20412121
Images
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Western blot - Anti-NRXN2 antibody (ab34245) Image courtesy of an anonymous Abreview.Anti-NRXN2 antibody (ab34245) at 1/400 dilution + whole cell lysate prepared from murine N2A cells at 20 µg
Secondary
Goat polyclonal conjugated to Dylight 800 at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 185 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
Samples were blocked using 0.2% casein for 1 hour at 37°C. -
Immunocytochemistry/ Immunofluorescence - Anti-NRXN2 antibody (ab34245)ICC/IF image of ab34245 stained SKNSH cells. The cells were 4% formaldehdye fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab34245, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.