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Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP13786] to NR0B1 / Dax1 - N-terminal
  • Suitable for: ICC/IF, IHC-P, WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal
    See all NR0B1 / Dax1 primary antibodies

  • Description

    Rabbit monoclonal [EP13786] to NR0B1 / Dax1 - N-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • A431 and Human testis lysates; Human testis tissue; SH-SY5Y cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649)
    Immunocytochemistry/ Immunofluorescence - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649)

    Immunofluorescent analysis of SH-SY5Y cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling NR0B1 / Dax1 with ab196649 at 1/300 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/400 dilution and counter-stained with DAPI (blue).

    Note: Nuclear and cytoplasm staining on SH-SY5Y cell line was observed.

  • Flow Cytometry - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649)
    Flow Cytometry - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649)

    Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labeling NR0B1 / Dax1 with purified ab196649 at 1:150 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077)(1:2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling NR0B1 / Dax1 with ab196649 at 1/1000 dilution  followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).

    Note: Nuclear staining on human testis tissue was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649)
    Western blot - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649)
    Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649) at 1/1000 dilution + Human testis lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 52 kDa

  • Western blot - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649)
    Western blot - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649)
    Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649) at 1/1000 dilution + A431 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 52 kDa

  • Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649)
    Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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