Anti-Noxa antibody (ab36833)
Key features and details
- Rabbit polyclonal to Noxa
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Noxa antibody
See all Noxa primary antibodies -
Description
Rabbit polyclonal to Noxa -
Host species
Rabbit -
Specificity
Although immunogen is from Mouse, customer feedback indicates that the results for Mouse are non-specific (see Abreviews). -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat
-
Immunogen
Synthetic peptide corresponding to 17 amino acids at the amino terminus of mouse Noxa. (Peptide available as ab39823.)
-
Positive control
- Human stomach tissue lysate.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Noxa antibody (ab36833)Anti-Noxa antibody (ab36833) at 0.5 µg/ml + Human stomach cell lysate
Predicted band size: 11.6 (mouse), 6 (human) kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Noxa antibody (ab36833)ab36833 at 1µg/ml staining Noxa in human stomach tissue by IHC
-
Immunocytochemistry/ Immunofluorescence - Anti-Noxa antibody (ab36833)ICC/IF image of ab36833 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab36833, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
-
Immunocytochemistry/ Immunofluorescence - Anti-Noxa antibody (ab36833)Immunofluorescence of Noxa in Human Stomach cells using ab36833 at 10 ug/ml.
