Anti-NOR1/TEC antibody (ab94507)
Key features and details
- Rabbit polyclonal to NOR1/TEC
- Suitable for: IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-NOR1/TEC antibody
See all NOR1/TEC primary antibodies -
Description
Rabbit polyclonal to NOR1/TEC -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Human, Pig
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Immunogen
Synthetic peptide corresponding to Human NOR1/TEC aa 200-300 conjugated to keyhole limpet haemocyanin.
(Peptide available asab107791) -
Positive control
- This antibody gave a positive signal in NIH3T3 whole cell lysate. FFPE mouse brain tissue sections.
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General notes
This product was previously labelled as NOR1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab94507 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. WB Use a concentration of 1 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). Target
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Function
Binds to the B1A response-element. -
Tissue specificity
Isoform alpha is highly expressed in skeletal muscle. Isoform beta is highly expressed in skeletal muscle and low expressed in fetal brain and placenta. -
Involvement in disease
Defects in NR4A3 are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving NR4A3 is found in patients with Erwing sarcoma. Translocation t(9;22)(q22-31;q11-12) with EWSR1.
Note=A chromosomal aberration involving NR4A3 is a cause of a form of extraskeletal myxoid chondrosarcomas (EMC). Translocation t(9;17)(q22;q11) with TAF2N. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR4 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 8013 Human
- Entrez Gene: 18124 Mouse
- Entrez Gene: 397479 Pig
- Entrez Gene: 58853 Rat
- Omim: 600542 Human
- SwissProt: Q92570 Human
- SwissProt: Q9QZB6 Mouse
- SwissProt: P51179 Rat
see all -
Alternative names
- CHN antibody
- Chondrosarcoma, extraskeletal myxoid, fused to EWS antibody
- CSMF antibody
see all
Images
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Western blot - Anti-NOR1/TEC antibody (ab94507)Anti-NOR1/TEC antibody (ab94507) at 1 µg/ml + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 68 kDa
Exposure time: 20 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOR1/TEC antibody (ab94507)IHC image of NOR1/TEC staining in mouse brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab84507, 1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
References (5)
ab94507 has been referenced in 5 publications.
- Ma C et al. The pro-inflammatory effect of NR4A3 in osteoarthritis. J Cell Mol Med 24:930-940 (2020). PubMed: 31701670
- Chen H et al. miR-106b-5p modulates acute pulmonary embolism via NOR1 in pulmonary artery smooth muscle cells. Int J Mol Med 45:1525-1533 (2020). PubMed: 32323756
- Ozair MZ et al. hPSC Modeling Reveals that Fate Selection of Cortical Deep Projection Neurons Occurs in the Subplate. Cell Stem Cell 23:60-73.e6 (2018). PubMed: 29937203
- Nagaoka M et al. The Orphan Nuclear Receptor NR4A3 Is Involved in the Function of Dendritic Cells. J Immunol 199:2958-2967 (2017). PubMed: 28893954
- Brunjes PC & Osterberg SK Developmental Markers Expressed in Neocortical Layers Are Differentially Exhibited in Olfactory Cortex. PLoS One 10:e0138541 (2015). PubMed: 26407299
Images
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Western blot - Anti-NOR1/TEC antibody (ab94507)Anti-NOR1/TEC antibody (ab94507) at 1 µg/ml + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 68 kDa
Exposure time: 20 minutes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOR1/TEC antibody (ab94507)
IHC image of NOR1/TEC staining in mouse brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab84507, 1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
