Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-nNav1.5 antibody [4G8:1G7] (ab62388)

Key features and details

  • Mouse monoclonal [4G8:1G7] to nNav1.5
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Human
  • Isotype: IgM

Overview

  • Product name

    Anti-nNav1.5 antibody [4G8:1G7]

  • Description

    Mouse monoclonal [4G8:1G7] to nNav1.5

  • Host species

    Mouse

  • Specificity

    The immunogen used was 14 amino acids long and only 6 are specific for the neonatal variant therefore it is possible it may also bind the adult splice variant.

  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide corresponding to Human nNav1.5.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-nNav1.5 antibody [4G8:1G7] (ab62388)
    Immunocytochemistry/ Immunofluorescence - Anti-nNav1.5 antibody [4G8:1G7] (ab62388)
    Ab62388 (10ug/ml) staining human Nav1.5 in Nav1.5-HEK293 recombinant cell line by immunocytochemistry.

    A: Plasma membrane localisation of Nav1.5 identified by ab62388 detected by anti-mouse Alexa Fluor-488 conjugated antibody.

    B: DAP1 co-staining of cell nuclei.
  • Flow Cytometry - Anti-nNav1.5 antibody [4G8:1G7] (ab62388)
    Flow Cytometry - Anti-nNav1.5 antibody [4G8:1G7] (ab62388)

    Overlay histogram showing SHSY-5Y cells stained with ab62388 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62388, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879>) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed SHSY-5Y cells used under the same conditions.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

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