Anti-nNav1.5 antibody [4G8:1G7] (ab62388)
![Anti-nNav1.5 antibody [4G8:1G7] (ab62388)](https://www.abcam.com/ps/products/62/ab62388/Images/ab62388-75718-anti-nnav15-antibody-4g81g7-immunofluorescence.jpg "Anti-nNav1.5 antibody [4G8:1G7] (ab62388)")
Key features and details
- Mouse monoclonal [4G8:1G7] to nNav1.5
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgM
Overview
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Product name
Anti-nNav1.5 antibody [4G8:1G7] -
Description
Mouse monoclonal [4G8:1G7] to nNav1.5 -
Host species
Mouse -
Specificity
The immunogen used was 14 amino acids long and only 6 are specific for the neonatal variant therefore it is possible it may also bind the adult splice variant. -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human nNav1.5.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
4G8:1G7 -
Isotype
IgM -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-nNav1.5 antibody [4G8:1G7] (ab62388)Ab62388 (10ug/ml) staining human Nav1.5 in Nav1.5-HEK293 recombinant cell line by immunocytochemistry.
A: Plasma membrane localisation of Nav1.5 identified by ab62388 detected by anti-mouse Alexa Fluor-488 conjugated antibody.
B: DAP1 co-staining of cell nuclei. -
![Flow Cytometry - Anti-nNav1.5 antibody [4G8:1G7] (ab62388)](https://www.abcam.com/ps/products/62/ab62388/Images/ab62388-75717-anti-nnav15-antibody-4g81g7-flow-cytometry.jpg)
Flow Cytometry - Anti-nNav1.5 antibody [4G8:1G7] (ab62388)Overlay histogram showing SHSY-5Y cells stained with ab62388 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62388, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879>) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed SHSY-5Y cells used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
