Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NG2 antibody [EPR23752-147] (ab259324)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23752-147] to NG2
  • Suitable for: Flow Cyt, IHC-P, ICC
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-NG2 antibody [EPR23752-147]
    See all NG2 primary antibodies

  • Description

    Rabbit monoclonal [EPR23752-147] to NG2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC
    Mouse
    Rat
    IHC-P
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse cerebrum tissue. ICC: Mouse primary neural/glia cells; Rat primary neural/glia cells. Flow cyt: NIH/3T3 and mouse primary neural glia cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NG2 antibody [EPR23752-147] (ab259324)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NG2 antibody [EPR23752-147] (ab259324)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling NG2 with ab259324 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebrum (PMID: 31769737). The section was incubated with ab259324 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

  • Immunocytochemistry - Anti-NG2 antibody [EPR23752-147] (ab259324)
    Immunocytochemistry - Anti-NG2 antibody [EPR23752-147] (ab259324)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neural/glia cells labelling NG2 with ab259324 at 1/100 dilution (4.82 ug/ml), followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/ml) (Green). Confocal image showing positive staining in mouse primary glia cells. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 ug/ml) (Red). The Nuclear counterstain was DAPI (Blue).

    -ve control 1: ab259324 at 1/50 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution.

    -ve control 2: ab11267 at 1/1000 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution.

  • Flow Cytometry - Anti-NG2 antibody [EPR23752-147] (ab259324)
    Flow Cytometry - Anti-NG2 antibody [EPR23752-147] (ab259324)

    Flow cytometric analysis of Mouse primary neural glia cells cells labelling NG2 with ab259324 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).

    Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Gated on viable cells.

  • Flow Cytometry - Anti-NG2 antibody [EPR23752-147] (ab259324)
    Flow Cytometry - Anti-NG2 antibody [EPR23752-147] (ab259324)

    Flow cytometric analysis of N9 (Mouse microglia, Left) / NIH/3T3 (Mouse embryonic fibroblast, Right) cells labelling NG2 with ab259324 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Negative control: N9 cells.

    Gated on viable cells.

  • Immunocytochemistry - Anti-NG2 antibody [EPR23752-147] (ab259324)
    Immunocytochemistry - Anti-NG2 antibody [EPR23752-147] (ab259324)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized rat primary neural/glia cells labelling NG2 with ab259324 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing positive staining in rat primary glia cells. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection.  ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain MAP2 at 1/500 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    -ve control 1: ab259324 at 1/50 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution.

    -ve control 2: ab11267 at 1/1000 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution.

  • Anti-NG2 antibody [EPR23752-147] (ab259324)
    Anti-NG2 antibody [EPR23752-147] (ab259324)

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