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Anti-NG2 antibody [7.1] - BSA and Azide free (ab279351)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [7.1] to NG2 - BSA and Azide free
  • Suitable for: Flow Cyt, ICC
  • Reacts with: Human

Overview

  • Product name

    Anti-NG2 antibody [7.1] - BSA and Azide free
    See all NG2 primary antibodies

  • Description

    Mouse monoclonal [7.1] to NG2 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cyt, ICCmore details
    Unsuitable for: WB

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC: SK-MEL-28 cells. Flow Cyt: SK-MEL-28 cells.

  • General notes

    ab279351 is the carrier-free version of ab279348. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab279351 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Flow Cytometry - Anti-NG2 antibody [7.1] - BSA and Azide free (ab279351)
    Flow Cytometry - Anti-NG2 antibody [7.1] - BSA and Azide free (ab279351)

    This data was developed using ab279348 the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of SK-BR-3 (Human breast adenocarcinoma epithelial cell, Left) / SK-MEL-28 (Human malignant melanoma, Right) cells labelling NG2 with ab279348 at 1/1000 dilution (0.1µg) (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.

    Negative control: SK-BR-3.

    Gated on viable cells.

  • Immunocytochemistry - Anti-NG2 antibody [7.1] - BSA and Azide free (ab279351)
    Immunocytochemistry - Anti-NG2 antibody [7.1] - BSA and Azide free (ab279351)

    This data was developed using ab279348 the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-MEL-28 cells labelling NG2 with ab279348 at 1/100 (11.02 µg/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in SK-MEL-28 cells.

    Negative control: SK-BR-3 (PMID: 20852124).

    ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Negative control 1: ab271294 at a 1/100 dilution followed by ab150080 at a 1/1000 dilution.

    Negative control 2: ab179513 at a 1/200 dilution followed by ab150157 at a 1/1000 dilution.

  • Anti-NG2 antibody [7.1] - BSA and Azide free (ab279351)
    Anti-NG2 antibody [7.1] - BSA and Azide free (ab279351)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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