Anti-NFS1 antibody (ab229829)
Key features and details
- Rabbit polyclonal to NFS1
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-NFS1 antibody
See all NFS1 primary antibodies -
Description
Rabbit polyclonal to NFS1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Orangutan
-
Immunogen
Recombinant fragment corresponding to Human NFS1 aa 200 to the C-terminus.
Database link: Q9Y697-1 -
Positive control
- WB: HepG2, HeLa and K562 whole cell lysates; Mouse kidney lysate. IHC-P: Human colon cancer tissue. IP: HeLa whole cell lysate.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-NFS1 antibody (ab229829)All lanes : Anti-NFS1 antibody (ab229829) at 1/1000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : Mouse kidney lysate
Lane 4 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFS1 antibody (ab229829)Paraffin-embedded human colon cancer tissue stained for NFS1 using ab229829 at 1/100 dilution in immunohistochemical analysis.
-
Immunoprecipitation - Anti-NFS1 antibody (ab229829)NFS1 was immunoprecipitated from 500 µg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 4 µg ab229829. An HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as secondary antibody at 1/50000 dilution.
Lane 1: Rabbit monoclonal IgG IP in HeLa whole cell lysate.
Lane 2: ab229829 IP in HeLa whole cell extract.
Lane 3: HeLa whole cell lysate 20 µg (Input).
