Anti-NFAT2 (phospho S237) antibody [EPR2377(N)] (ab183023)
![Anti-NFAT2 (phospho S237) antibody [EPR2377(N)] (ab183023)](https://www.abcam.com/ps/products/183/ab183023/Images/ab183023-215339-anti-nfat2-phospho-s237-antibody-epr2377n-western-blot.jpg "Anti-NFAT2 (phospho S237) antibody [EPR2377(N)] (ab183023)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2377(N)] to NFAT2 (phospho S237)
- Suitable for: Flow Cyt, WB
- Reacts with: Human
Overview
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Product name
Anti-NFAT2 (phospho S237) antibody [EPR2377(N)]
See all NFAT2 primary antibodies -
Description
Rabbit monoclonal [EPR2377(N)] to NFAT2 (phospho S237) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HEK-293 cell lysates untreated and treated with Ionomycin and TPA
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2377(N) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NFAT2 (phospho S237) antibody [EPR2377(N)] (ab183023)All lanes : Anti-NFAT2 (phospho S237) antibody [EPR2377(N)] (ab183023) at 1/10000 dilution
Lane 1 : HEK-293 lysate treated with Ionomycin and TPA
Lane 2 : HEK-293 lysate treated with Ionomycin and TPA, then treated with alkaline phosphatase
Lane 3 : HEK-293 lysate untreated
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 101 kDa
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![Flow Cytometry - Anti-NFAT2 (phospho S237) antibody [EPR2377(N)] (ab183023)](https://www.abcam.com/ps/products/183/ab183023/Images/ab183023-215340-anti-nfat2-phospho-s237-antibody-epr2377n-flow-cytometry.jpg)
Flow Cytometry - Anti-NFAT2 (phospho S237) antibody [EPR2377(N)] (ab183023)Flow cytometry analysis of permealized 293 cells labeling NFAT2 (phospho S237) using ab183023 at 1/200 dilution (red). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG (green).
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![Anti-NFAT2 (phospho S237) antibody [EPR2377(N)] (ab183023)](https://www.abcam.com/ps/products/183/ab183023/Images/ab183023-3-benefits-of-recombinant-antibodies.png)
Anti-NFAT2 (phospho S237) antibody [EPR2377(N)] (ab183023)
