Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Neurogranin antibody [NG22] - BSA and Azide free (ab273465)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [NG22] to Neurogranin - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Neurogranin antibody [NG22] - BSA and Azide free
    See all Neurogranin primary antibodies

  • Description

    Mouse monoclonal [NG22] to Neurogranin - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC or IHC-Fr

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human brain lysate. IHC-P: Human cerebrum tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab273465 is the carrier-free version of ab273444. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab273465 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-Neurogranin antibody - BSA and Azide free (ab273465)
    Western blot - Anti-Neurogranin antibody - BSA and Azide free (ab273465)
    All lanes : Anti-Neurogranin antibody [NG22] (ab273444) at 1/1000 dilution

    Lane 1 : Human brain lysate
    Lane 2 : Human lung lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 8 kDa



    This data was developed using ab273444 the same antibody clone in a different buffer formulation.

    Negative control: Human lung (PMID: 20665622).
    The molecular weight observed is consistent with what has been described in the literature (PMID: 20875798).

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody - BSA and Azide free (ab273465)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody - BSA and Azide free (ab273465)

    This data was developed using ab273444 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Neurogranin with ab273444 at 1/10000 (0.111 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Positive staining on human cererbum. The section was incubated with ab273444 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody - BSA and Azide free (ab273465)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody - BSA and Azide free (ab273465)

    This data was developed using ab273444 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human lung tissue labeling Neurogranin with ab273444 at 1/10000 (0.111 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Negative control: No staining on human lung. The section was incubated with ab273444 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Anti-Neurogranin antibody [NG22] - BSA and Azide free (ab273465)
    Anti-Neurogranin antibody [NG22] - BSA and Azide free (ab273465)

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