Anti-Neurogranin antibody [NG22] - BSA and Azide free (ab273465)
![Anti-Neurogranin antibody [NG22] - BSA and Azide free (ab273465)](https://www.abcam.com/ps/products/273/ab273465/Images/ab273465-413360-anti-neurogranin-antibody-ng22-western-blot-human-brain10-ug.jpg "Anti-Neurogranin antibody [NG22] - BSA and Azide free (ab273465)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [NG22] to Neurogranin - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Neurogranin antibody [NG22] - BSA and Azide free
See all Neurogranin primary antibodies -
Description
Mouse monoclonal [NG22] to Neurogranin - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC or IHC-Fr -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human brain lysate. IHC-P: Human cerebrum tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
ab273465 is the carrier-free version of ab273444. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab273465 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
NG22 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Neurogranin antibody - BSA and Azide free (ab273465)All lanes : Anti-Neurogranin antibody [NG22] (ab273444) at 1/1000 dilution
Lane 1 : Human brain lysate
Lane 2 : Human lung lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 8 kDaThis data was developed using ab273444 the same antibody clone in a different buffer formulation.
Negative control: Human lung (PMID: 20665622).
The molecular weight observed is consistent with what has been described in the literature (PMID: 20875798).Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody - BSA and Azide free (ab273465)This data was developed using ab273444 the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Neurogranin with ab273444 at 1/10000 (0.111 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Positive staining on human cererbum. The section was incubated with ab273444 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody - BSA and Azide free (ab273465)This data was developed using ab273444 the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human lung tissue labeling Neurogranin with ab273444 at 1/10000 (0.111 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Negative control: No staining on human lung. The section was incubated with ab273444 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
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![Anti-Neurogranin antibody [NG22] - BSA and Azide free (ab273465)](https://www.abcam.com/ps/products/273/ab273465/Images/ab273465-4-benefits-of-recombinant-antibodies.png)
Anti-Neurogranin antibody [NG22] - BSA and Azide free (ab273465)
