Anti-NELF antibody [EPR8976(2)] - BSA and Azide free (ab250641)
![Anti-NELF antibody [EPR8976(2)] - BSA and Azide free (ab250641)](https://www.abcam.com/ps/products/250/ab250641/Images/ab250641-2-anti-nelf-antibody-epr8976-2-bsa-and-azide-free-flow-cytometry.jpg "Anti-NELF antibody [EPR8976(2)] - BSA and Azide free (ab250641)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8976(2)] to NELF - BSA and Azide free
- Suitable for: Flow Cyt, WB
- Reacts with: Human
Overview
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Product name
Anti-NELF antibody [EPR8976(2)] - BSA and Azide free
See all NELF primary antibodies -
Description
Rabbit monoclonal [EPR8976(2)] to NELF - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250641 is the carrier-free version of ab183022. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250641 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8976(2) -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-NELF antibody [EPR8976(2)] - BSA and Azide free (ab250641)This data was developed using ab183022, the same antibody clone in a different buffer formulation.Flow cytometry analysis of PC3 cells labeling NELF using ab183022 at 1/300 dilution. A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG.
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![Western blot - Anti-NELF antibody [EPR8976(2)] - BSA and Azide free (ab250641)](https://www.abcam.com/ps/products/250/ab250641/Images/ab250641-398082-anti-nelf-antibody-epr8976-2-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-NELF antibody [EPR8976(2)] - BSA and Azide free (ab250641)All lanes : Anti-NELF antibody [EPR8976(2)] (ab183022) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Human colon cancer lysate
Lane 4 : Human testis lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 60 kDaThis data was developed using ab183022, the same antibody clone in a different buffer formulation.
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![Anti-NELF antibody [EPR8976(2)] - BSA and Azide free (ab250641)](https://www.abcam.com/ps/products/250/ab250641/Images/ab250641-3-benefits-of-recombinant-antibodies.png)
Anti-NELF antibody [EPR8976(2)] - BSA and Azide free (ab250641)