Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NEK2 antibody [20/NEK2] - BSA and Azide free (ab279722)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [20/NEK2] to NEK2 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-NEK2 antibody [20/NEK2] - BSA and Azide free
    See all NEK2 primary antibodies

  • Description

    Mouse monoclonal [20/NEK2] to NEK2 - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat; K-562 whole cell lysates.

  • General notes

    ab279722 is the carrier-free version of ab279717. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab279722 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-NEK2 antibody [20/NEK2] - BSA and Azide free (ab279722)
    Western blot - Anti-NEK2 antibody [20/NEK2] - BSA and Azide free (ab279722)
    All lanes : Anti-NEK2 antibody [20/NEK2] (ab279717) at 1/1000 dilution

    Lane 1 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
    Lane 2 : HFF-1 (human Skin fibroblast), whole cell lysate
    Lane 3 : K-562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 52 kDa
    Observed band size: 48 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab279717, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression molecular weight observed is consistent with what has been described in the literature (PMID:25043295).

    Negative control: HFF-1 (PMID:27509921).

    Exposure time: 48 seconds

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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