Anti-NEK2 antibody [20/NEK2] (ab279717)
![Anti-NEK2 antibody [20/NEK2] (ab279717)](https://www.abcam.com/ps/products/279/ab279717/Images/ab279717-1-anti-nek2-antibody-20nek2-western-blot-jurkat.jpg "Anti-NEK2 antibody [20/NEK2] (ab279717)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [20/NEK2] to NEK2
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-NEK2 antibody [20/NEK2]
See all NEK2 primary antibodies -
Description
Mouse monoclonal [20/NEK2] to NEK2 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Jurkat; K-562 whole cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
20/NEK2 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-NEK2 antibody [20/NEK2] (ab279717)All lanes : Anti-NEK2 antibody [20/NEK2] (ab279717) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 2 : HFF-1 (human Skin fibroblast), whole cell lysate
Lane 3 : K-562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression molecular weight observed is consistent with what has been described in the literature (PMID:25043295).
Negative control: HFF-1 (PMID:27509921).
Exposure time: 48 seconds