Anti-Nectin-4 antibody [EPR15613-68] - BSA and Azide free (ab251110)
![Anti-Nectin-4 antibody [EPR15613-68] - BSA and Azide free (ab251110)](https://www.abcam.com/ps/products/251/ab251110/Images/ab251110-402998-anti-nectin-4-antibody-epr15613-68-bsa-and-azide-free-western-blot.jpg "Anti-Nectin-4 antibody [EPR15613-68] - BSA and Azide free (ab251110)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15613-68] to Nectin-4 - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
-
Product name
Anti-Nectin-4 antibody [EPR15613-68] - BSA and Azide free
See all Nectin-4 primary antibodies -
Description
Rabbit monoclonal [EPR15613-68] to Nectin-4 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
-
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
General notes
Ab251110 is the carrier-free version of ab192033. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251110 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15613-68 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Nectin-4 antibody [EPR15613-68] - BSA and Azide free (ab251110)All lanes : Anti-Nectin-4 antibody [EPR15613-68] (ab192033) at 1/1000 dilution
Lane 1 : T47D lysate
Lane 2 : MCF7 lysate
Lane 3 : Human placenta lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 55 kDaThis data was developed using ab192033, the same antibody clone in a different buffer formulation.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin-4 antibody [EPR15613-68] - BSA and Azide free (ab251110)](https://www.abcam.com/ps/products/251/ab251110/Images/ab251110-1-anti-nectin-4-antibody-epr15613-68-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin-4 antibody [EPR15613-68] - BSA and Azide free (ab251110)This data was developed using ab192033, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling Nectin-4 with ab192033 at 1/3000 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
Inset: Negative control: using PBS instead of primary antibody. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
![Anti-Nectin-4 antibody [EPR15613-68] - BSA and Azide free (ab251110)](https://www.abcam.com/ps/products/251/ab251110/Images/ab251110-3-benefits-of-recombinant-antibodies.png)
Anti-Nectin-4 antibody [EPR15613-68] - BSA and Azide free (ab251110)