Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NDUFS2 antibody [EPR16266] (ab192022)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16266] to NDUFS2
  • Suitable for: WB, IHC-P, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-NDUFS2 antibody [EPR16266]
    See all NDUFS2 primary antibodies

  • Description

    Rabbit monoclonal [EPR16266] to NDUFS2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Rat
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, Jurkat, 293T cell lysate. Mouse brain, kidney and spleen lysate. Rat brain and kidney lysate. C6, RAW264.7, PC-12 and NIH/3T3 cell lysate. Human brain tissue. Rat stomach tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-NDUFS2 antibody [EPR16266] (ab192022)
    Western blot - Anti-NDUFS2 antibody [EPR16266] (ab192022)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: NDUFS2 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: Jurkat whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab192022 observed at 48 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab192022 was shown to specifically react with NDUFS2 in wild-type HAP1 cells whilst signal was lost in NDUFS2 knockout cells. Wild-type and NDUFS2 knockout samples were subjected to SDS-PAGE. ab192022 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS2 antibody [EPR16266] (ab192022)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS2 antibody [EPR16266] (ab192022)

    Immunohistochemical analysis of paraffin-embedded human brain tissue sections labeling NDUFS2 using ab192022 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Negative control uses PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-NDUFS2 antibody [EPR16266] (ab192022)
    Immunoprecipitation - Anti-NDUFS2 antibody [EPR16266] (ab192022)

    Lane 1: 293T cell lysate was immunoprecipitated using ab192022 at a 1/40 dilution. Secondary used was anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at a 1/1500 dilution.

    Lane 2: PBS instead of 293T lysates.

  • Western blot - Anti-NDUFS2 antibody [EPR16266] (ab192022)
    Western blot - Anti-NDUFS2 antibody [EPR16266] (ab192022)
    All lanes : Anti-NDUFS2 antibody [EPR16266] (ab192022) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : 293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 53 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS2 antibody [EPR16266] (ab192022)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS2 antibody [EPR16266] (ab192022)

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue sections labeling NDUFS2 using ab192022 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Negative control uses PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-NDUFS2 antibody [EPR16266] (ab192022)
    Western blot - Anti-NDUFS2 antibody [EPR16266] (ab192022)
    All lanes : Anti-NDUFS2 antibody [EPR16266] (ab192022) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : Mouse spleen lysate
    Lane 4 : Rat brain lysate
    Lane 5 : Rat kidney lysate
    Lane 6 : C6 cell lysate
    Lane 7 : RAW264.7 cell lysate
    Lane 8 : PC-12 cell lysate
    Lane 9 : NIH3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 53 kDa
    Observed band size: 49 kDa
    why is the actual band size different from the predicted?

  • Anti-NDUFS2 antibody [EPR16266] (ab192022)
    Anti-NDUFS2 antibody [EPR16266] (ab192022)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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