Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Tuesday March 02, 2021

Anti-NDUFS2 antibody [EPR16266] (ab192022)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16266] to NDUFS2
Suitable for: WB, IHC-P, IP
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-NDUFS2 antibody [EPR16266]
See all NDUFS2 primary antibodies
Description

Rabbit monoclonal [EPR16266] to NDUFS2
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Rat Human
IP	Human
WB	Mouse Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- HeLa, Jurkat, 293T cell lysate. Mouse brain, kidney and spleen lysate. Rat brain and kidney lysate. C6, RAW264.7, PC-12 and NIH/3T3 cell lysate. Human brain tissue. Rat stomach tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16266
Isotype

IgG
Research areas

Western blot - Anti-NDUFS2 antibody [EPR16266] (ab192022)

Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: NDUFS2 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: Jurkat whole cell lysate (20 µg)

Lanes 1 - 4: Merged signal (red and green). Green - ab192022 observed at 48 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab192022 was shown to specifically react with NDUFS2 in wild-type HAP1 cells whilst signal was lost in NDUFS2 knockout cells. Wild-type and NDUFS2 knockout samples were subjected to SDS-PAGE. ab192022 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS2 antibody [EPR16266] (ab192022)

Immunohistochemical analysis of paraffin-embedded human brain tissue sections labeling NDUFS2 using ab192022 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Negative control uses PBS instead of primary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-NDUFS2 antibody [EPR16266] (ab192022)

Lane 1: 293T cell lysate was immunoprecipitated using ab192022 at a 1/40 dilution. Secondary used was anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at a 1/1500 dilution.
Lane 2: PBS instead of 293T lysates.
Western blot - Anti-NDUFS2 antibody [EPR16266] (ab192022)

All lanes : Anti-NDUFS2 antibody [EPR16266] (ab192022) at 1/10000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : 293T cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 53 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS2 antibody [EPR16266] (ab192022)

Immunohistochemical analysis of paraffin-embedded rat stomach tissue sections labeling NDUFS2 using ab192022 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Negative control uses PBS instead of primary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-NDUFS2 antibody [EPR16266] (ab192022)

All lanes : Anti-NDUFS2 antibody [EPR16266] (ab192022) at 1/1000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat brain lysate
Lane 5 : Rat kidney lysate
Lane 6 : C6 cell lysate
Lane 7 : RAW264.7 cell lysate
Lane 8 : PC-12 cell lysate
Lane 9 : NIH3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 53 kDa
Observed band size: 49 kDa
why is the actual band size different from the predicted?
Anti-NDUFS2 antibody [EPR16266] (ab192022)

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