Anti-Nck antibody [5B7] (ab129367)
![Anti-Nck antibody [5B7] (ab129367)](https://www.abcam.com/ps/products/129/ab129367/Images/ab129367-143550-anti-nck-antibody-5b7-western-blot.jpg "Anti-Nck antibody [5B7] (ab129367)")
Key features and details
- Mouse monoclonal [5B7] to Nck
- Suitable for: WB, ELISA, IHC-P, Flow Cyt
- Reacts with: Human, African green monkey, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-Nck antibody [5B7]
See all Nck primary antibodies -
Description
Mouse monoclonal [5B7] to Nck -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB HumanRecombinant fragment
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Immunogen
Purified recombinant fragment of Human Nck expressed in E. coli(203-371)
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Positive control
- Jurkat, HeLa, HEK293, A431, K562 and COS7 cell lysates; Jurkat cells. Human rectum cancer tissue; Human Nck (aa203-371) recombinant protein.
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General notes
This product was changed from ascites to supernatant. Lot no’s high than GR133551-6 are from Tissue Culture Supernatant
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
5B7 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Nck antibody [5B7] (ab129367)All lanes : Anti-Nck antibody [5B7] (ab129367) at 1/500 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HEK293 cell lysate
Lane 4 : A431 cell lysate
Lane 5 : K562 cell lysate
Lane 6 : Cos7 cell lysate
Predicted band size: 43 kDa
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![ELISA - Anti-Nck antibody [5B7] (ab129367)](https://www.abcam.com/ps/products/129/ab129367/Images/ab129367-143553-anti-nck-antibody-5b7-elisa.jpg)
ELISA - Anti-Nck antibody [5B7] (ab129367)ab129367 staining Nck antigen by ELISA. -
![Flow Cytometry - Anti-Nck antibody [5B7] (ab129367)](https://www.abcam.com/ps/products/129/ab129367/Images/ab129367-143552-anti-nck-antibody-5b7-flow-cytometry.jpg)
Flow Cytometry - Anti-Nck antibody [5B7] (ab129367)ab129367, at 1/200 dilution, staining Nck in Jurkat cells by flow cytometry (green). Negative control shown in red. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nck antibody [5B7] (ab129367)](https://www.abcam.com/ps/products/129/ab129367/Images/ab129367-143551-anti-nck-antibody-5b7-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nck antibody [5B7] (ab129367)ab129367, at 1/200 dilution, staining Nck in paraffin-embedded Human rectum cancer tissue by Immunohistochemistry, with DAB staining. -
![Western blot - Anti-Nck antibody [5B7] (ab129367)](https://www.abcam.com/ps/products/129/ab129367/Images/ab129367-143549-anti-nck-antibody-5b7-western-blot.jpg)
Western blot - Anti-Nck antibody [5B7] (ab129367)Anti-Nck antibody [5B7] (ab129367) at 1/500 dilution + Human Recombinant Nck fragment (aa 203-371)
Predicted band size: 43 kDa
The expected molecular weight of the recombinant fragment is 45 kDa.
