Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-WAVE 1 antibody [EPR15021] - N-terminal (ab185546)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15021] to WAVE 1 - N-terminal
  • Suitable for: Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-WAVE 1 antibody [EPR15021] - N-terminal
    See all WAVE 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR15021] to WAVE 1 - N-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • U87-MG, 293T and K562 cell lysate. K562 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-WAVE 1 antibody [EPR15021] - N-terminal (ab185546)
    Western blot - Anti-WAVE 1 antibody [EPR15021] - N-terminal (ab185546)
    All lanes : Anti-WAVE 1 antibody [EPR15021] - N-terminal (ab185546) at 1/10000 dilution

    Lane 1 : U87-MG cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : 293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 61 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?

  • Flow Cytometry - Anti-WAVE 1 antibody [EPR15021] - N-terminal (ab185546)
    Flow Cytometry - Anti-WAVE 1 antibody [EPR15021] - N-terminal (ab185546)

    Flow Cytometry analysis of K562 cells labeling WAVE 1 using ab185546 at a 1/200 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.

  • Anti-WAVE 1 antibody [EPR15021] - N-terminal (ab185546)
    Anti-WAVE 1 antibody [EPR15021] - N-terminal (ab185546)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-WAVE 1 antibody [EPR15021] - N-terminal (ab185546)

Clear all

Recently viewed products

© 2021 Astana Biomed Group LLP. All rights reserved.