Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Mutant p53 antibody [Y5] - BSA and Azide free (ab219731)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y5] to Mutant p53 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-Mutant p53 antibody [Y5] - BSA and Azide free
    See all Mutant p53 primary antibodies

  • Description

    Rabbit monoclonal [Y5] to Mutant p53 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: A431 cells. WB: A431 lysate. ICC: A431 cells. IHC-P: Bladder, Skin Cancer, Glioma, Gastric adenocarcinoma, Breast carcinoma tissue. Flow Cyt: A431 cells.

  • General notes

    ab219731 is the carrier-free version of ab32049.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mutant p53 antibody [Y5] - BSA and Azide free (ab219731)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mutant p53 antibody [Y5] - BSA and Azide free (ab219731)

    Immunohistochemical analysis of paraffin embedded normal Human uterus tissue (negative control) labeling p53 with ab32049.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32049).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-Mutant p53 antibody [Y5] - BSA and Azide free (ab219731)
    Flow Cytometry - Anti-Mutant p53 antibody [Y5] - BSA and Azide free (ab219731)

    Flow Cytometry analysis of A431(human epidermoid carcinoma) cells labeling Mutant p53 with unpurified ab32049 at 1/20 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32049).

  • Immunocytochemistry/ Immunofluorescence - Anti-Mutant p53 antibody [Y5] - BSA and Azide free (ab219731)
    Immunocytochemistry/ Immunofluorescence - Anti-Mutant p53 antibody [Y5] - BSA and Azide free (ab219731)

    Confocal image showing nuclear staining on A431 cell line.

    Immunocytochemistry/Immunofluorescence analysis of A431(Human epidermoid carcinoma cell line) labelling Mutant p53 with ab32049 at 1/100. Cells were fixed with 4% Paraformaldehyde (20 minutes) and permeabilized with 0.1% Triton X-100 (5 minutes). ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counterstained with ab195889, anti- alpha tubulin antibody (1/200) using an Alexa Fluor® 594-conjugated microtubule marker as the secondary. Nuclei were counterstained with DAPI (blue).

    Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32049).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mutant p53 antibody [Y5] - BSA and Azide free (ab219731)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mutant p53 antibody [Y5] - BSA and Azide free (ab219731)

    Immunohistochemical analysis of paraffin embedded normal Human breast tissue (negative control) labeling p53 with ab32049.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32049).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mutant p53 antibody [Y5] - BSA and Azide free (ab219731)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mutant p53 antibody [Y5] - BSA and Azide free (ab219731)

    Immunohistochemistry (Paraffin-embedded sections) using ab32049 at a dilution of 1/50 and human skin cancer

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32049).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mutant p53 antibody [Y5] - BSA and Azide free (ab219731)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mutant p53 antibody [Y5] - BSA and Azide free (ab219731)

    ab32049 showing positive staining in Glioma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32049).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mutant p53 antibody [Y5] - BSA and Azide free (ab219731)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mutant p53 antibody [Y5] - BSA and Azide free (ab219731)

    ab32049 showing positive staining in Gastric adenocarcinoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32049).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mutant p53 antibody [Y5] - BSA and Azide free (ab219731)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mutant p53 antibody [Y5] - BSA and Azide free (ab219731)

    ab32049 showing positive staining in Urinary bladder carcinoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32049).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mutant p53 antibody [Y5] - BSA and Azide free (ab219731)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mutant p53 antibody [Y5] - BSA and Azide free (ab219731)

    ab32049 showing positive staining in Breast carcinoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32049).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-Mutant p53 antibody [Y5] - BSA and Azide free (ab219731)
    OI-RD Scanning - Anti-Mutant p53 antibody [Y5] - BSA and Azide free (ab219731)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32049).

  • Anti-Mutant p53 antibody [Y5] - BSA and Azide free (ab219731)
    Anti-Mutant p53 antibody [Y5] - BSA and Azide free (ab219731)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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