Anti-CD11b antibody [EPR19373] - BSA and Azide free (ab250744)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19373] to CD11b - BSA and Azide free
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-CD11b antibody [EPR19373] - BSA and Azide free
See all CD11b primary antibodies -
Description
Rabbit monoclonal [EPR19373] to CD11b - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WBmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250744 is the carrier-free version of ab184307. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250744 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19373 -
Isotype
IgG -
Research areas
Images
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This data was developed using ab184307, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde-fixed RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukaemia virus) cell line labeling CD11b with ab184307 at 1/120 dilution (red) compared with a Rabbit IgG,monoclonal [EPR25A]- Isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; (blue). Goat anti Rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.
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This data was developed using ab184307, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde-fixed WEHI-231 (Mouse lymphoblast B cell lymphoma cell line) cells labeling CD11b with ab184307 at 1/120 dilution (red) compared with a Rabbit IgG,monoclonal [EPR25A]-Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody. Negative control: WEHI-231 is negative for CD11b.
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Lanes 1-3 : Anti-CD11b antibody [EPR19373] (ab184307) at 1/1000 dilution
Lanes 4-5 : Anti-CD11b antibody [EPR19373] (ab184307) at 1/5000 dilution
Lane 1 : Mouse spleen lysate
Lane 2 : M1 (Mouse myeloblast myeloid leukaemia cell line) whole cell lysate
Lane 3 : WEHI-231 (Mouse lymphoblast B cell lymphoma cell line) whole cell lysate
Lane 4 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 5 : J774A.1 (Mouse macrophage reticulum cell sarcoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 127 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab184307, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
CD11b is a 170 kD glycoprotein also known as aM integrin, Mac-1 a subunit (PMID: 10603370). It is exclusively restricted to cells of the myeloid lineages and monocytes, but not in T or B cell lines, so WEHI-231 cell lysate serves as a negative control (PMID: 2457584).
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All lanes : Anti-CD11b antibody [EPR19373] (ab184307) at 1/1000 dilution
Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : Rat spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 127 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab184307, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
CD11b is a 170 kD glycoprotein also known as α integrin, Mac-1 α subunit (PMID: 10603370).
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