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Signal Transduction Cytoskeleton / ECM Cell Adhesion Integrins Alpha

Anti-CD11b antibody [EPR19373] - BSA and Azide free (ab250744)

Price and availability

526 012 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-CD11b antibody [EPR19373] - BSA and Azide free (ab250744)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19373] to CD11b - BSA and Azide free
  • Suitable for: Flow Cyt, WB
  • Reacts with: Mouse, Rat

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Overview

  • Product name

    Anti-CD11b antibody [EPR19373] - BSA and Azide free
    See all CD11b primary antibodies
  • Description

    Rabbit monoclonal [EPR19373] to CD11b - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250744 is the carrier-free version of ab184307. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250744 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19373
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Integrins
    • Alpha
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Glial
    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Microglia marker
    • Stem Cells
    • Mesenchymal Stem Cells
    • Negative Markers
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Murine Lineage Negative
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • NK Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Neutrophil Lineage

Images

  • Flow Cytometry - Anti-CD11b antibody [EPR19373] - BSA and Azide free (ab250744)
    Flow Cytometry - Anti-CD11b antibody [EPR19373] - BSA and Azide free (ab250744)
    This data was developed using ab184307, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde-fixed RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukaemia virus) cell line labeling CD11b with ab184307 at 1/120 dilution (red) compared with a Rabbit IgG,monoclonal [EPR25A]- Isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; (blue). Goat anti Rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.
  • Flow Cytometry - Anti-CD11b antibody [EPR19373] - BSA and Azide free (ab250744)
    Flow Cytometry - Anti-CD11b antibody [EPR19373] - BSA and Azide free (ab250744)
    This data was developed using ab184307, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde-fixed WEHI-231 (Mouse lymphoblast B cell lymphoma cell line) cells labeling CD11b with ab184307 at 1/120 dilution (red) compared with a Rabbit IgG,monoclonal [EPR25A]-Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody. Negative control: WEHI-231 is negative for CD11b.
  • Western blot - Anti-CD11b antibody [EPR19373] - BSA and Azide free (ab250744)
    Western blot - Anti-CD11b antibody [EPR19373] - BSA and Azide free (ab250744)
    Lanes 1-3 : Anti-CD11b antibody [EPR19373] (ab184307) at 1/1000 dilution
    Lanes 4-5 : Anti-CD11b antibody [EPR19373] (ab184307) at 1/5000 dilution

    Lane 1 : Mouse spleen lysate
    Lane 2 : M1 (Mouse myeloblast myeloid leukaemia cell line) whole cell lysate
    Lane 3 : WEHI-231 (Mouse lymphoblast B cell lymphoma cell line) whole cell lysate
    Lane 4 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 5 : J774A.1 (Mouse macrophage reticulum cell sarcoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 127 kDa
    Observed band size: 170 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab184307, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    CD11b is a 170 kD glycoprotein also known as aM integrin, Mac-1 a subunit (PMID: 10603370). It is exclusively restricted to cells of the myeloid lineages and monocytes, but not in T or B cell lines, so WEHI-231 cell lysate serves as a negative control (PMID: 2457584).

  • Western blot - Anti-CD11b antibody [EPR19373] - BSA and Azide free (ab250744)
    Western blot - Anti-CD11b antibody [EPR19373] - BSA and Azide free (ab250744)
    All lanes : Anti-CD11b antibody [EPR19373] (ab184307) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 2 : Rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 127 kDa
    Observed band size: 170 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab184307, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    CD11b is a 170 kD glycoprotein also known as α integrin, Mac-1 α subunit (PMID: 10603370).

  • Anti-CD11b antibody [EPR19373] - BSA and Azide free (ab250744)
    Anti-CD11b antibody [EPR19373] - BSA and Azide free (ab250744)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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