Anti-Mus81 antibody [MTA30 2G10/3] (ab14387)
![Anti-Mus81 antibody [MTA30 2G10/3] (ab14387)](https://www.abcam.com/ps/products/14/ab14387/Images/ab14387-21896-anti-mus81-antibody-mta30-2g10-3-western-blot.jpg "Anti-Mus81 antibody [MTA30 2G10/3] (ab14387)")
Key features and details
- Mouse monoclonal [MTA30 2G10/3] to Mus81
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-Mus81 antibody [MTA30 2G10/3]
See all Mus81 primary antibodies -
Description
Mouse monoclonal [MTA30 2G10/3] to Mus81 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Mus81 protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
from TCS -
Clonality
Monoclonal -
Clone number
MTA30 2G10/3 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG2a -
Research areas
Images
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Western blot - Anti-Mus81 antibody [MTA30 2G10/3] (ab14387)Anti-Mus81 antibody [MTA30 2G10/3] (ab14387) at 1/1000 dilution + HeLa cell lysate
Predicted band size: 66 kDa
Observed band size: 64 kDa why is the actual band size different from the predicted?
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![Flow Cytometry - Anti-Mus81 antibody [MTA30 2G10/3] (ab14387)](https://www.abcam.com/ps/products/14/ab14387/Images/ab14387-21895-anti-mus81-antibody-mta30-2g10-3-flow-cytometry.jpg)
Flow Cytometry - Anti-Mus81 antibody [MTA30 2G10/3] (ab14387)Overlay histogram showing HeLa cells stained with ab14387 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14387, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.