Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MUC2 antibody [EPR6145] - BSA and Azide free (ab226154)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6145] to MUC2 - BSA and Azide free
  • Suitable for: WB, IHC-P, ELISA
  • Reacts with: Human

Overview

  • Product name

    Anti-MUC2 antibody [EPR6145] - BSA and Azide free
    See all MUC2 primary antibodies

  • Description

    Rabbit monoclonal [EPR6145] to MUC2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ELISAmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Caco2, SKBR3, HT1376, SH SY5Y and SK OV3 cell lysates and human small intestine and colon lysates. IHC-P: Human colon tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    ab226154 is the carrier-free version of ab134119. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] - BSA and Azide free (ab226154)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] - BSA and Azide free (ab226154)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling MUC2 with purified ab134119 at 1/15,000 dilution (0.01 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab134119).

  • ELISA - Anti-MUC2 antibody [EPR6145] - BSA and Azide free (ab226154)
    ELISA - Anti-MUC2 antibody [EPR6145] - BSA and Azide free (ab226154)

    This data was developed using ab134119, the same antibody clone in a different buffer formulation.

    Indirect ELISA using ab134119 at varying antibody concentrations (1000-0 ng/mL) and MUC2 antigen at 1000 ng/mL. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as a secondary antibody.

  • Anti-MUC2 antibody [EPR6145] - BSA and Azide free (ab226154)
    Anti-MUC2 antibody [EPR6145] - BSA and Azide free (ab226154)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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