Anti-MUC2 antibody [EPR6145] - BSA and Azide free (ab226154)
![Anti-MUC2 antibody [EPR6145] - BSA and Azide free (ab226154)](https://www.abcam.com/ps/products/226/ab226154/Images/ab226154-385411-antimuc2antibodyepr6145immunohistochemistrycolonhuman.jpg "Anti-MUC2 antibody [EPR6145] - BSA and Azide free (ab226154)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6145] to MUC2 - BSA and Azide free
- Suitable for: WB, IHC-P, ELISA
- Reacts with: Human
Overview
-
Product name
Anti-MUC2 antibody [EPR6145] - BSA and Azide free
See all MUC2 primary antibodies -
Description
Rabbit monoclonal [EPR6145] to MUC2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ELISAmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Caco2, SKBR3, HT1376, SH SY5Y and SK OV3 cell lysates and human small intestine and colon lysates. IHC-P: Human colon tissue.
-
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
ab226154 is the carrier-free version of ab134119. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6145 -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] - BSA and Azide free (ab226154)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling MUC2 with purified ab134119 at 1/15,000 dilution (0.01 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab134119).
-
![ELISA - Anti-MUC2 antibody [EPR6145] - BSA and Azide free (ab226154)](https://www.abcam.com/ps/products/226/ab226154/Images/ab226154-451052-anti-muc2-antibody-epr6145-elisa-muc25.png)
ELISA - Anti-MUC2 antibody [EPR6145] - BSA and Azide free (ab226154)This data was developed using ab134119, the same antibody clone in a different buffer formulation.
Indirect ELISA using ab134119 at varying antibody concentrations (1000-0 ng/mL) and MUC2 antigen at 1000 ng/mL. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as a secondary antibody.
-
![Anti-MUC2 antibody [EPR6145] - BSA and Azide free (ab226154)](https://www.abcam.com/ps/products/226/ab226154/Images/ab226154-4-benefits-of-recombinant-antibodies.png)
Anti-MUC2 antibody [EPR6145] - BSA and Azide free (ab226154)
