Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MUC1 antibody [EPR1023] - Low endotoxin, Azide free (ab194978)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1023] to MUC1 - Low endotoxin, Azide free
  • Suitable for: IHC-P, WB, IP, Flow Cyt, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MUC1 antibody [EPR1023] - Low endotoxin, Azide free
    See all MUC1 primary antibodies

  • Description

    Rabbit monoclonal [EPR1023] to MUC1 - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Specificity

    ab194978 detects the full-length MUC1 and the 17 kDa carboxy-terminal subunit (subunit beta).

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Rat
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • T47-D cell lysate, colon cancer lysate, Human breast carcinoma tissue, Human normal breast tissue.

  • General notes

    ab194978 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [EPR1023] - Low endotoxin, Azide free (ab194978)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [EPR1023] - Low endotoxin, Azide free (ab194978)

    Immunohistochemical staining of paraffin embedded rat kidney with purified ab109185 at a working dilution of 1 in 500. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109185).

  • Immunoprecipitation - Anti-MUC1 antibody [EPR1023] - Low endotoxin, Azide free (ab194978)
    Immunoprecipitation - Anti-MUC1 antibody [EPR1023] - Low endotoxin, Azide free (ab194978)

    ab109185 (purified) at 1/20 immunoprecipitating MUC1 in T47-D (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109185).

  • Flow Cytometry - Anti-MUC1 antibody [EPR1023] - Low endotoxin, Azide free (ab194978)
    Flow Cytometry - Anti-MUC1 antibody [EPR1023] - Low endotoxin, Azide free (ab194978)

    Overlay histogram showing MCF7 cells fixed in 2% PFA and stained with purified ab109185 at a dilution of 1 in 30 (pink line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 150. Rabbit monoclonal IgG was used as an isotype control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109185).

  • Immunocytochemistry/ Immunofluorescence - Anti-MUC1 antibody [EPR1023] - Low endotoxin, Azide free (ab194978)
    Immunocytochemistry/ Immunofluorescence - Anti-MUC1 antibody [EPR1023] - Low endotoxin, Azide free (ab194978)

    Immunofluorescence staining of A431 cells with purified ab109185 at a working dilution of 1 in 1000, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 555 goat anti rabbit, used at a dilution of 1 in 400. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, purified ab109185 was used at a dilution of 1/200 followed by an Alexa Fluor® 555 goat anti-mouse antibody at a dilution of 1/500.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109185).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [EPR1023] - Low endotoxin, Azide free (ab194978)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [EPR1023] - Low endotoxin, Azide free (ab194978)

    Immunohistochemical staining of paraffin embedded mouse lung with purified ab109185 at a working dilution of 1 in 500. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109185).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [EPR1023] - Low endotoxin, Azide free (ab194978)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [EPR1023] - Low endotoxin, Azide free (ab194978)

    Immunohistochemical staining of paraffin embedded human endometrium with purified ab109185 at a working dilution of 1 in 500. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109185).

  • Flow Cytometry - Anti-MUC1 antibody [EPR1023] - Low endotoxin, Azide free (ab194978)
    Flow Cytometry - Anti-MUC1 antibody [EPR1023] - Low endotoxin, Azide free (ab194978)

    Overlay histogram showing MCF7 cells stained with unpurified ab109185 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109185, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109185).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [EPR1023] - Low endotoxin, Azide free (ab194978)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [EPR1023] - Low endotoxin, Azide free (ab194978)

    ab109185 (unpurified) showing positive staining in Normal stomach tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109185).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-MUC1 antibody [EPR1023] - Low endotoxin, Azide free (ab194978)
    OI-RD Scanning - Anti-MUC1 antibody [EPR1023] - Low endotoxin, Azide free (ab194978)

    Equilibrium disassociation constant (KD) of unpurified ab109185.
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109185).

  • Western blot - Anti-MUC1 antibody [EPR1023] - Low endotoxin, Azide free (ab194978)
    Western blot - Anti-MUC1 antibody [EPR1023] - Low endotoxin, Azide free (ab194978)
    All lanes : Anti-MUC1 antibody [EPR1023] (ab109185) at 1/1000 dilution

    Lane 1 : Wild-type HeLa whole cell lysate
    Lane 2 : MUC1 knockout HeLa whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 122 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab194978).

    Lanes 1 - 2: Merged signal (red and green). Green - ab109185 observed at 24 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab109185 was shown to specifically react with MUC1 in wild-type HeLa cells as signal was lost in MUC1 knockout cells. Wild-type and MUC1 knockout samples were subjected to SDS-PAGE. Ab109185 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [EPR1023] - Low endotoxin, Azide free (ab194978)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [EPR1023] - Low endotoxin, Azide free (ab194978)

    This IHC data was generated using the same anti-MUC1 antibody clone [EPR1023] in a different buffer formulation (cat# ab109185).

    ab109185 (unpurified) showing positive staining in Breast ductal infiltrating carcinoma tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-MUC1 antibody [EPR1023] - Low endotoxin, Azide free (ab194978)
    Anti-MUC1 antibody [EPR1023] - Low endotoxin, Azide free (ab194978)

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