Anti-MTA2/PID antibody (ab8106)
Key features and details
- Rabbit polyclonal to MTA2/PID
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-MTA2/PID antibody
See all MTA2/PID primary antibodies -
Description
Rabbit polyclonal to MTA2/PID -
Host species
Rabbit -
Specificity
No cross-reactivity to MTA1. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human MTA2/PID aa 652-668.
Sequence:PAPSHPASTNEPIVLED
(Peptide available asab6243) -
General notes
This product was previously labelled as MTA2
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-MTA2/PID antibody (ab8106)All lanes : Anti-MTA2/PID antibody (ab8106) at 1 µg/ml
Lane 1 : HeLa whole cell lysate with absence of blocking peptide
Lane 2 : HeLa whole cell lysate with presence of blocking peptide
Predicted band size: 75 kDa
-
Immunocytochemistry/ Immunofluorescence - Anti-MTA2/PID antibody (ab8106)ab8106 at 10µg/ml staining MTA2/PID in Hela cells by ICC/IF
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTA2/PID antibody (ab8106)ab8106 (2µg/ml) staining MTA2/PID in human ileum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
Immunocytochemistry/ Immunofluorescence - Anti-MTA2/PID antibody (ab8106)Immunofluorescence of PID in HeLa cells using ab8106 at 10 ug/ml.
