Anti-HDAC6 antibody [178] (ab253032)
Key features and details
- Mouse monoclonal [178] to HDAC6
- Suitable for: ELISA, ICC/IF
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-HDAC6 antibody [178]
See all HDAC6 primary antibodies -
Description
Mouse monoclonal [178] to HDAC6 -
Host species
Mouse -
Tested applications
Suitable for: ELISA, ICC/IFmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Full length protein corresponding to Human HDAC6.
Database link: Q9UBN7 -
Epitope
The antibody detects an epitope in the ZnF domain of HDAC6. -
Positive control
- ICC/IF: HEK-293T cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Affinity purified from hybridoma culture supernatant. -
Clonality
Monoclonal -
Clone number
178 -
Isotype
IgG -
Research areas
Images
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Formaldehyde-fixed, Triton-permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling HDAC6 (Green) using ab253032 at 25 µg/mL. Coverslips were incubated with an anti-mouse Ab conjugated to AlexaFluor®488. Slides were counterstained with DAPI (Blue).
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Detection of human HDAC6 using ab253032 by ELISA. The plate was coated with recombinant human HDAC6 protein. BSA-blocked plate was probed with a dilution series of ab253032 (10-4 - 103 nM) followed by incubation with anti-mouse IgG secondary antibody conjugated to HRP.