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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation

Anti-HDAC6 antibody [178] (ab253032)

Anti-HDAC6 antibody [178] (ab253032)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [178] to HDAC6
  • Suitable for: ELISA, ICC/IF
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG

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Overview

  • Product name

    Anti-HDAC6 antibody [178]
    See all HDAC6 primary antibodies
  • Description

    Mouse monoclonal [178] to HDAC6
  • Host species

    Mouse
  • Tested applications

    Suitable for: ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Human, Recombinant fragment
  • Immunogen

    Full length protein corresponding to Human HDAC6.
    Database link: Q9UBN7

  • Epitope

    The antibody detects an epitope in the ZnF domain of HDAC6.
  • Positive control

    • ICC/IF: HEK-293T cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Affinity purified from hybridoma culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    178
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • HDACs
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HDACs
    • Class II / Hda1 Class

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-HDAC6 antibody [178] (ab253032)
    Immunocytochemistry/ Immunofluorescence - Anti-HDAC6 antibody [178] (ab253032)

    Formaldehyde-fixed, Triton-permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling HDAC6 (Green) using ab253032 at 25 µg/mL. Coverslips were incubated with an anti-mouse Ab conjugated to AlexaFluor®488. Slides were counterstained with DAPI (Blue).

  • ELISA - Anti-HDAC6 antibody [178] (ab253032)
    ELISA - Anti-HDAC6 antibody [178] (ab253032)

    Detection of human HDAC6 using ab253032 by ELISA. The plate was coated with recombinant human HDAC6 protein. BSA-blocked plate was probed with a dilution series of ab253032 (10-4 - 103 nM) followed by incubation with anti-mouse IgG secondary antibody conjugated to HRP.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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