Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MSK2 / RSK-B antibody (ab42101)

Key features and details

  • Rabbit polyclonal to MSK2 / RSK-B
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab42101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Human
All applications
Rat
Application Abreviews Notes
WB (2)
Use a concentration of 1 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
ICC/IF
Use a concentration of 1 - 5 µg/ml.
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
ICC/IF
Use a concentration of 1 - 5 µg/ml.

Target

  • Function

    Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. Essential role in the control of RELA transcriptional activity in response to TNF. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF) and result in the transcriptional activation of several immediate early genes, including proto-oncogenes FOS and JUN (By similarity). Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).

  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 2 protein kinase domains.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • 90 kDa ribosomal protein S6 kinase 4 antibody
      • EC 2.7.11.1 antibody
      • KS6A4_HUMAN antibody
      • Mitogen and stress activated protein kinase 2 antibody
      • Nuclear mitogen and stress activated protein kinase 2 antibody
      • Nuclear mitogen- and stress-activated protein kinase 2 antibody
      • Ribosomal protein kinase B antibody
      • Ribosomal protein S6 kinase 90kD polypeptide 4 antibody
      • Ribosomal protein S6 kinase 90kDa polypeptide 4 antibody
      • Ribosomal protein S6 kinase alpha 4 antibody
      • Ribosomal protein S6 kinase alpha-4 antibody
      • RPS6KA4 antibody
      • RSKB antibody
      • S6K-alpha-4 antibody
      see all

    Images

    • Western blot - Anti-MSK2 / RSK-B antibody (ab42101)
      Western blot - Anti-MSK2 / RSK-B antibody (ab42101)
      All lanes : Anti-MSK2 / RSK-B antibody (ab42101) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
      Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size: 86 kDa
      Observed band size: 86 kDa
      Additional bands at: 150 kDa, 50 kDa, 70 kDa. We are unsure as to the identity of these extra bands.

    • Immunocytochemistry/ Immunofluorescence - Anti-MSK2 / RSK-B antibody (ab42101)
      Immunocytochemistry/ Immunofluorescence - Anti-MSK2 / RSK-B antibody (ab42101)
      ICC/IF image of ab42101 stained MCF7 cells. The cells were 4%PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42101, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, Hek293 and HepG2 cells fixed in 4%PFA at 1ug/ml and HeLa, Hek293, HepG2 and MCF7 cells fixed in 100% methanol at 5ug/ml.

    References (2)

    Publishing research using ab42101? Please let us know so that we can cite the reference in this datasheet.

    ab42101 has been referenced in 2 publications.

    • Kang JI & Ahn BY HCV-induced PKR activation is stimulated by the mitogen- and stress-activated protein kinase MSK2. Biochem Biophys Res Commun 407:248-53 (2011). WB ; Human . PubMed: 21385567
    • Raymond B  et al. Anthrax lethal toxin impairs IL-8 expression in epithelial cells through inhibition of histone H3 modification. PLoS Pathog 5:e1000359 (2009). WB ; Human . PubMed: 19343203

    Images

    • Western blot - Anti-MSK2 / RSK-B antibody (ab42101)
      Western blot - Anti-MSK2 / RSK-B antibody (ab42101)
      All lanes : Anti-MSK2 / RSK-B antibody (ab42101) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
      Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size: 86 kDa
      Observed band size: 86 kDa
      Additional bands at: 150 kDa, 50 kDa, 70 kDa. We are unsure as to the identity of these extra bands.

    • Immunocytochemistry/ Immunofluorescence - Anti-MSK2 / RSK-B antibody (ab42101)
      Immunocytochemistry/ Immunofluorescence - Anti-MSK2 / RSK-B antibody (ab42101)
      ICC/IF image of ab42101 stained MCF7 cells. The cells were 4%PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42101, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, Hek293 and HepG2 cells fixed in 4%PFA at 1ug/ml and HeLa, Hek293, HepG2 and MCF7 cells fixed in 100% methanol at 5ug/ml.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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