Anti-MPP4 antibody (ab106848)
Key features and details
- Rabbit polyclonal to MPP4
- Suitable for: IHC-P, WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-MPP4 antibody -
Description
Rabbit polyclonal to MPP4 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse
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Immunogen
Synthetic peptide corresponding to Rat MPP4 aa 400 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab130918) -
Positive control
- This antibody gave a positive signal in Rat Retina tissue lysate and in FFPE rat eye tissue sections.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab106848 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-P Use at an assay dependent concentration. WB Use a concentration of 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 72 kDa). Target
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Function
May play a role in retinal photoreceptors development. -
Tissue specificity
Expressed in the retina (at protein level). Highly expressed in the retina. Lower amounts are detected in brain, testis, ARPE-19, RPE/choroid and fetal eye. Isoform 5 is retina-specific. -
Sequence similarities
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 2 L27 domains.
Contains 1 PDZ (DHR) domain.
Contains 1 SH3 domain. -
Cellular localization
Cytoplasm. Detected at the outer limiting membrane (OLM) and in the outer plexiform layer (OPL) of the retina. At the OLM, detected apical to the adherens junction. - Information by UniProt
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Database links
- Entrez Gene: 227157 Mouse
- Entrez Gene: 58808 Rat
- SwissProt: Q6P7F1 Mouse
- SwissProt: Q9QYH1 Rat
- Unigene: 474049 Mouse
- Unigene: 163075 Rat
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Alternative names
- ALS2CR5 antibody
- Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 5 protein antibody
- Discs large homolog 6 antibody
see all
Images
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Western blot - Anti-MPP4 antibody (ab106848)Anti-MPP4 antibody (ab106848) at 1 µg/ml (Milk blocking - 5%) + Rat Retina Tissue Lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 72 kDa
Observed band size: 73 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutes
The predicted molecular weight of MPP4 is 50 kDa (SwissProt), however we expect to observe a banding pattern at 73 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPP4 antibody (ab106848)This image is courtesy of an Abreview submitted by Carl Hobbsab106848 staining mouse retina sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab106848 at 1/4000 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPP4 antibody (ab106848)This image is courtesy of an Abreview submitted by Carl Hobbsab106848 staining rat retina sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab106848 at 1/4000 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPP4 antibody (ab106848)IHC image of ab106848 staining in rat eye formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab106848, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
References (0)
ab106848 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-MPP4 antibody (ab106848)Anti-MPP4 antibody (ab106848) at 1 µg/ml (Milk blocking - 5%) + Rat Retina Tissue Lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 72 kDa
Observed band size: 73 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutes
The predicted molecular weight of MPP4 is 50 kDa (SwissProt), however we expect to observe a banding pattern at 73 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPP4 antibody (ab106848) This image is courtesy of an Abreview submitted by Carl Hobbs
ab106848 staining mouse retina sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab106848 at 1/4000 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPP4 antibody (ab106848) This image is courtesy of an Abreview submitted by Carl Hobbs
ab106848 staining rat retina sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab106848 at 1/4000 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MPP4 antibody (ab106848)IHC image of ab106848 staining in rat eye formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab106848, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.