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Anti-MNDA antibody [253A] (ab188566)

Key features and details

  • Mouse monoclonal [253A] to MNDA
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-MNDA antibody [253A]
    See all MNDA primary antibodies

  • Description

    Mouse monoclonal [253A] to MNDA

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein (His-tag) corresponding to Human MNDA aa 1 to the C-terminus. The immunogen was also conjugated to a proprietary tag.
    Database link: Q08619

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • V5-tagged MNDA expressed in Hek293T, Hek-v5-MNDA, Z138, MINO and MT-2 cell lines. Human tonsil, lymph node and kidney tissue..

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-MNDA antibody [253A] (ab188566)
    Western blot - Anti-MNDA antibody [253A] (ab188566)
    All lanes : Anti-MNDA antibody [253A] (ab188566)

    Lane 1 : Hek-v5-MNDA cell line (Positive)
    Lane 2 : Hek-v5-CD1D cell line (Negative control)
    Lane 3 : HL60 cell line (Negative)
    Lane 4 : K562 cell line (Negative)
    Lane 5 : Z138 cell line (Positive)
    Lane 6 : MINO cell line (Positive)
    Lane 7 : YT cell line (Negative)
    Lane 8 : MT-2 cell line (Positive)

    Lysates/proteins at 100 µg per lane.

    Predicted band size: 47 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MNDA antibody [253A] (ab188566)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MNDA antibody [253A] (ab188566)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling MNDA with ab188566 undiluted.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MNDA antibody [253A] (ab188566)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MNDA antibody [253A] (ab188566)

    Immunohistochemical analysis of paraffin-embedded Human lymph node tissue labeling MNDA with ab188566 undiluted.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MNDA antibody [253A] (ab188566)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MNDA antibody [253A] (ab188566)

    Immunohistochemical analysis of paraffin-embedded Human Tonsil tissue labeling MNDA with ab188566 undiluted.

  • Immunocytochemistry/ Immunofluorescence - Anti-MNDA antibody [253A] (ab188566)
    Immunocytochemistry/ Immunofluorescence - Anti-MNDA antibody [253A] (ab188566)

    Left Panel: Immunocytochemistry analysis of V5-tagged MNDA expressed in Hek293T labeling MNDA with ab188566

    Middle Panel: Shows labeling with the anti-V5 to confirm the efficiency of the transfection.

    Right Panel: Preparation of NANOG transfected cells used as a negative control.

     Frozen cytospins preparations were used for the analysis

  • Immunocytochemistry/ Immunofluorescence - Anti-MNDA antibody [253A] (ab188566)
    Immunocytochemistry/ Immunofluorescence - Anti-MNDA antibody [253A] (ab188566)

    Immunofluorescent analysis of Human tonsil labeling MNDA with ab188566 undiluted (green)

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