Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µg 328 339 ₸

Order now and get it on Thursday March 04, 2021

Key features and details

Mouse monoclonal [253A] to MNDA
Suitable for: IHC-P, WB, ICC/IF
Reacts with: Human
Isotype: IgG1

Product name

Anti-MNDA antibody [253A]
See all MNDA primary antibodies
Description

Mouse monoclonal [253A] to MNDA
Host species

Mouse

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant full length protein (His-tag) corresponding to Human MNDA aa 1 to the C-terminus. The immunogen was also conjugated to a proprietary tag.
Database link: Q08619

Run BLAST with Run BLAST with
Positive control
- V5-tagged MNDA expressed in Hek293T, Hek-v5-MNDA, Z138, MINO and MT-2 cell lines. Human tonsil, lymph node and kidney tissue..
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
Purity

Protein G purified
Clonality

Monoclonal
Clone number

253A
Isotype

IgG1
Research areas

Western blot - Anti-MNDA antibody [253A] (ab188566)

All lanes : Anti-MNDA antibody [253A] (ab188566)

Lane 1 : Hek-v5-MNDA cell line (Positive)
Lane 2 : Hek-v5-CD1D cell line (Negative control)
Lane 3 : HL60 cell line (Negative)
Lane 4 : K562 cell line (Negative)
Lane 5 : Z138 cell line (Positive)
Lane 6 : MINO cell line (Positive)
Lane 7 : YT cell line (Negative)
Lane 8 : MT-2 cell line (Positive)

Lysates/proteins at 100 µg per lane.

Predicted band size: 47 kDa
Observed band size: 50 kDa
why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MNDA antibody [253A] (ab188566)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling MNDA with ab188566 undiluted.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MNDA antibody [253A] (ab188566)

Immunohistochemical analysis of paraffin-embedded Human lymph node tissue labeling MNDA with ab188566 undiluted.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MNDA antibody [253A] (ab188566)

Immunohistochemical analysis of paraffin-embedded Human Tonsil tissue labeling MNDA with ab188566 undiluted.
Immunocytochemistry/ Immunofluorescence - Anti-MNDA antibody [253A] (ab188566)

Left Panel: Immunocytochemistry analysis of V5-tagged MNDA expressed in Hek293T labeling MNDA with ab188566
Middle Panel: Shows labeling with the anti-V5 to confirm the efficiency of the transfection.
Right Panel: Preparation of NANOG transfected cells used as a negative control.
Frozen cytospins preparations were used for the analysis
Immunocytochemistry/ Immunofluorescence - Anti-MNDA antibody [253A] (ab188566)

Immunofluorescent analysis of Human tonsil labeling MNDA with ab188566 undiluted (green)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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